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Journal of Clinical Microbiology, August 2000, p. 3106-3109, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Identification of Paracoccidioides brasiliensis by PCR Amplification of Ribosomal DNA

Andrea B. Motoyama,1,dagger Emerson J. Venancio,1,2 Gilberto O. Brandão,1 Silvana Petrofeza-Silva,3 Ildinete S. Pereira,1 Celia M. A. Soares,3 and Maria Sueli S. Felipe1,*

Laboratório de Biologia Molecular, Instituto de Biologia, Universidade de Brasília, 70910-900, Brasília, DF,1 Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970, Goiânia, GO,3 and Departamento de Ciências Patológicas, Universidade Estadual de Londrina, 86051-990, Londrina, PR,2 Brazil

Received 28 January 2000/Returned for modification 29 February 2000/Accepted 1 June 2000

We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and molecular epidemiological studies of P. brasiliensis in Latin America.

* Corresponding author. Mailing address: Depto. de Biologia Celular, Instituto de Biologia, Universidade de Brasilia, Campus Universitario Darcy Ribeiro - Asa Norte, CEP 70910-900, Brasilia - DF, Brazil. Phone: 55 021 61 3072423. Fax: 55 021 61 3498411. E-mail: msueli{at}unb.br.

dagger Present address: Friedrich Miescher Institut Basel, Basel, Switzerland.

Journal of Clinical Microbiology, August 2000, p. 3106-3109, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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