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Journal of Clinical Microbiology, August 2000, p. 3116-3118, Vol. 38, No. 8
Mayo Clinic and Foundation, Rochester,
Minnesota 55905
Received 27 January 2000/Returned for modification 6 April
2000/Accepted 1 June 2000
Five hundred specimens (288 genital, 192 dermal, and 20 ocular)
were extracted by technologists, and the DNA was assayed by LightCycler
PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by
conventional tube and shell vial cell culture. One hundred fifty-eight
confirmed (by cell culture and TK target PCR) positive and
LightCycler-positive specimens were detected during the first 30 PCR
cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK).
LightCycler PCR is more sensitive (n = 197; 23.1%)
than cell cultures (n = 150) for the routine
laboratory detection of herpes simplex virus infections.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evaluation of LightCycler PCR for Implementation of
Laboratory Diagnosis of Herpes Simplex Virus Infections
*
Corresponding author. Mailing address: Division of
Clinical Microbiology, Mayo Clinic, 200 First St. SW, Rochester, MN
55905. Phone: (507) 284-8146. Fax: (507) 284-4272. E-mail:
tfsmith{at}mayo.edu.
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