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Journal of Clinical Microbiology, September 2000, p. 3205-3208, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Three Different Sensitive Assays for Hepatitis B Virus DNA in Monitoring of Responses to Antiviral Therapy

Henry L. Y. Chan, Nancy W. Y. Leung, Tracy C. M. Lau, May L. Wong, and Joseph J. Y. Sung*

Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong

Received 13 October 1999/Returned for modification 3 April 2000/Accepted 4 May 2000

The aim of our study was to compare the performances of two new hepatitis B virus (HBV) DNA assays, a cross-linking assay (NAXCOR) and a hybrid-capture amplification assay (Digene), versus the widely used branched-DNA (bDNA) assay (Chiron) in the monitoring of HBV DNA levels during antiviral treatment. Serial serum samples from 12 chronically HBV infected patients undergoing a phase II trial of an antiviral drug, 2',3'-dideoxy-5-fluoro-3'-thiacytidine (FTC), were studied. A total of 96 serum samples were tested for HBV DNA using the cross-linking, hybrid-capture amplification, and bDNA assays. In the comparison of the cross-linking and bDNA assays, concordant results were found in 77 (80.3%) samples, no significant difference was found between the median log10 HBV DNA levels (6.66 versus 7.17 meq/ml), and the results of the two assays were closely correlated (r = 0.95). In the comparison of the hybrid-capture amplification and bDNA assays, concordant results were found in 79 (82.3%) samples, no significant difference was found between the median log10 HBV DNA levels (6.98 versus 6.99 meq/ml), and the results of the two assays were closely correlated (r = 0.99). Six (6.3%) samples by the cross-linking assay and 10 (10.4%) samples by the bDNA assay required retesting because of unacceptably high within-run coefficients of variance. No sample required retesting in the hybrid-capture amplification assay according to the internal validation. In conclusion, the cross-linking and hybrid-capture amplification assays were as sensitive as the bDNA assay for HBV DNA detection and can be recommended for monitoring of HBV DNA levels during antiviral treatment.

* Corresponding author. Mailing address: Department of Medicine and Therapeutics, 9/F Prince of Wales Hospital, Shatin, New Territories, Hong Kong. Phone: (852) 2632-3132. Fax: (852) 2646-7824. E-mail: Joesung{at}cuhk.edu.hk.

Journal of Clinical Microbiology, September 2000, p. 3205-3208, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chan, H. L.-Y., Leung, N. W.-Y., Hui, A. Y., Wong, V. W.-S., Liew, C.-T., Chim, A. M.-L., Chan, F. K.-L., Hung, L. C.-T., Lee, Y.-T., Tam, J. S.-L., Lam, C. W.-K., Sung, J. J.-Y. (2005). A Randomized, Controlled Trial of Combination Therapy for Chronic Hepatitis B: Comparing Pegylated Interferon-{alpha}2b and Lamivudine with Lamivudine Alone. ANN INTERN MED 142: 240-250 [Abstract] [Full Text]  
  • Dai, C-Y, Yu, M-L, Chen, S-C, Lin, Z-Y, Hsieh, M-Y, Wang, L-Y, Tsai, J-F, Chuang, W-L, Chang, W-Y (2004). Clinical evaluation of the COBAS Amplicor HBV monitor test for measuring serum HBV DNA and comparison with the Quantiplex branched DNA signal amplification assay in Taiwan. J. Clin. Pathol. 57: 141-145 [Abstract] [Full Text]  
  • Chan, H. L.-Y., Wong, M. L., Hui, A. Y., Hung, L. C.-T., Chan, F. K.-L., Sung, J. J.-Y. (2003). Hepatitis B Virus Genotype C Takes a More Aggressive Disease Course Than Hepatitis B Virus Genotype B in Hepatitis B e Antigen-Positive Patients. J. Clin. Microbiol. 41: 1277-1279 [Abstract] [Full Text]  


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