Preclinical Diagnosis of Scrapie by Immunohistochemistry of Third Eyelid Lymphoid Tissue

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by O'Rourke, K. I.
	Articles by Knowles, D. P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by O'Rourke, K. I.
	Articles by Knowles, D. P.

Previous Article | Next Article

Journal of Clinical Microbiology, September 2000, p. 3254-3259, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Preclinical Diagnosis of Scrapie by Immunohistochemistry of Third Eyelid Lymphoid Tissue

K. I. O'Rourke,¹^,^* T. V. Baszler,²^,³ T. E. Besser,² J. M. Miller,⁴ R. C. Cutlip,⁴ G. A. H. Wells,⁵ S. J. Ryder,⁵ S. M. Parish,⁶ A. N. Hamir,⁴ N. E. Cockett,⁷ A. Jenny,⁸ and D. P. Knowles¹^,²

Animal Disease Research Unit, Animal Research Service, U.S. Department of Agriculture,¹ Department of Veterinary Microbiology and Pathology² and Department of Clinical Veterinary Sciences,⁶ College of Veterinary Medicine, Washington State University, and Washington Animal Disease Diagnostic Laboratory,³ Pullman, Washington; National Animal Disease Center, Agricultural Research Service,⁴ and National Veterinary Services Laboratories, Animal and Plant Health Inspection Service,⁸ U.S. Department of Agriculture, Ames, Iowa; Veterinary Laboratories Agency, Ministry of Agriculture, Fisheries, and Food, Surrey, United Kingdom⁵; and Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah⁷

Received 13 January 2000/Returned for modification 18 April 2000/Accepted 1 June 2000

Ovine scrapie is a member of the transmissible spongiform encephalopathies (TSEs), a heterogeneous family of fatal neurologicdisorders characterized by deposition of an abnormal isoform (prionprotein [PrP] PrP-Sc) of a cellular sialoglycoprotein in neuraltissue. PrP-Sc is detectable in some lymphoid tissues of infectedsheep months or years before development of clinical disease.Detection of PrP-Sc in these tissues is the basis for live-animaltesting. In this study, we characterize the performance of a preclinicaldiagnostic test for ovine scrapie based on a monoclonal antibody(MAb)-based immunohistochemistry assay of nictitating membrane("third eyelid")-associated lymphoid tissue. The results of thirdeyelid immunohistochemistry assay agreed with the scrapie statusof the sheep for 41 of 42 clinical suspects with confirmed scrapieand 174 of 175 sheep without scrapie. Third eyelid sampling agreedwith the scrapie status for 36 of 41 clinically normal sheep positivefor PrP-Sc immunostaining of brain tissue, including 27 sheepwith positive biopsy specimens that progressed to clinical diseasewith confirmed scrapie 3 to 20 months after biopsy. The assayused MAb F89/160.1.5, which binds to residues 142 to 145 of ovinePrP. This antibody can be used in combination with MAb F99/97.6.1,which binds to residues 220 to 225. One or both MAbs in this cocktailrecognize PrP sequences conserved in most mammalian species inwhich natural TSEs have been reported. Immunohistochemistry assayof routinely formalin-fixed lymphoid tissues with a cocktail ofpan-specific MAbs is a practical, readily standardized live-animaland preclinical test for ovinescrapie.

^* Corresponding author. Mailing address: USDA, ARS, ADRU, 3003 ADBF, P.O. Box 646630, Pullman, WA 99164-6630. Phone: (509) 335-6020.Fax: (509) 335-8328. E-mail: korourke{at}vetmed.wsu.edu.

Journal of Clinical Microbiology, September 2000, p. 3254-3259, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Manning, L., O'Rourke, K. I., Knowles, D. P., Marsh, S. A., Spencer, Y. I., Moffat, E., Wells, G. A.H., Czub, S. (2008). A collaborative Canadian-United Kingdom evaluation of an immunohistochemistry protocol to diagnose bovine spongiform encephalopathy. jvdi 20: 504-508 [Abstract] [Full Text]
Hamir, A. N., Richt, J. A., Miller, J. M., Kunkle, R. A., Hall, S. M., Nicholson, E. M., O'Rourke, K. I., Greenlee, J. J., Williams, E. S. (2008). Experimental Transmission of Chronic Wasting Disease (CWD) of Elk (Cervus elaphus nelsoni), White-tailed Deer (Odocoileus virginianus), and Mule Deer (Odocoileus hemionus hemionus) to White-tailed Deer by Intracerebral Route. Vet Pathol 45: 297-306 [Abstract] [Full Text]
Harrington, R. D., Baszler, T. V., O'Rourke, K. I., Schneider, D. A., Spraker, T. R., Liggitt, H. D., Knowles, D. P. (2008). A species barrier limits transmission of chronic wasting disease to mink (Mustela vison). J. Gen. Virol. 89: 1086-1096 [Abstract] [Full Text]
Gonzalez, L., Dagleish, M. P., Martin, S., Dexter, G., Steele, P., Finlayson, J., Jeffrey, M. (2008). Diagnosis of preclinical scrapie in live sheep by the immunohistochemical examination of rectal biopsies. Vet Rec. 162: 397-403 [Abstract] [Full Text]
Andrievskaia, O., Algire, J., Balachandran, A., Nielsen, K. (2008). Prion protein in sheep urine. jvdi 20: 141-146 [Abstract] [Full Text]
Gonzalez, L., Horton, R., Ramsay, D., Toomik, R., Leathers, V., Tonelli, Q., Dagleish, M. P., Jeffrey, M., Terry, L. (2008). Adaptation and evaluation of a rapid test for the diagnosis of sheep scrapie in samples of rectal mucosa. jvdi 20: 203-208 [Abstract] [Full Text]
Gavier-Widen, D., Noremark, M., Langeveld, J. P.M., Stack, M., Biacabe, A.-G., Vulin, J., Chaplin, M., Richt, J. A., Jacobs, J., Acin, C., Monleon, E., Renstrom, L., Klingeborn, B., Baron, T. G.M. (2008). Bovine spongiform encephalopathy in Sweden: an H-type variant. jvdi 20: 2-10 [Abstract] [Full Text]
Keane, D. P., Barr, D. J., Keller, J. E., Hall, S. M., Langenberg, J. A., Bochsler, P. N. (2008). Comparison of retropharyngeal lymph node and obex region of the brainstem in detection of chronic wasting disease in white-tailed deer (Odocoileus virginianus). jvdi 20: 58-60 [Abstract] [Full Text]
Hamir, A. N., Kunkle, R. A., Richt, J. A., Miller, J. M., Greenlee, J. J. (2008). Experimental Transmission of US Scrapie Agent by Nasal, Peritoneal, and Conjunctival Routes to Genetically Susceptible Sheep. Vet Pathol 45: 7-11 [Abstract] [Full Text]
Arnold, M. E., Ryan, J. B. M., Konold, T., Simmons, M. M., Spencer, Y. I., Wear, A., Chaplin, M., Stack, M., Czub, S., Mueller, R., Webb, P. R., Davis, A., Spiropoulos, J., Holdaway, J., Hawkins, S. A. C., Austin, A. R., Wells, G. A. H. (2007). Estimating the temporal relationship between PrPSc detection and incubation period in experimental bovine spongiform encephalopathy of cattle. J. Gen. Virol. 88: 3198-3208 [Abstract] [Full Text]
Wolfe, L. L., Spraker, T. R., Gonzalez, L., Dagleish, M. P., Sirochman, T. M., Brown, J. C., Jeffrey, M., Miller, M. W. (2007). PrPCWD in rectal lymphoid tissue of deer (Odocoileus spp.). J. Gen. Virol. 88: 2078-2082 [Abstract] [Full Text]
Hamir, A. N., Miller, J. M., Kunkle, R. A., Hall, S. M., Richt, J. A. (2007). Susceptibility of Cattle to First-passage Intracerebral Inoculation with Chronic Wasting Disease Agent from White-tailed Deer. Vet Pathol 44: 487-493 [Abstract] [Full Text]
Meade-White, K., Race, B., Trifilo, M., Bossers, A., Favara, C., Lacasse, R., Miller, M., Williams, E., Oldstone, M., Race, R., Chesebro, B. (2007). Resistance to Chronic Wasting Disease in Transgenic Mice Expressing a Naturally Occurring Allelic Variant of Deer Prion Protein. J. Virol. 81: 4533-4539 [Abstract] [Full Text]
Baeten, L. A., Powers, B. E., Jewell, J. E., Spraker, T. R., Miller, M. W. (2007). A Natural Case of Chronic Wasting Disease in a Free-ranging Moose (Alces alces shirasi). J Wildl Dis 43: 309-314 [Abstract] [Full Text]
Gretzschel, A., Buschmann, A., Langeveld, J., Groschup, M. H. (2006). Immunological characterization of abnormal prion protein from atypical scrapie cases in sheep using a panel of monoclonal antibodies. J. Gen. Virol. 87: 3715-3722 [Abstract] [Full Text]
Spraker, T. R., Gidlewski, T. L., Balachandran, A., VerCauteren, K. C., Creekmore, L., Munger, R. D. (2006). Detection of PrPCWD in postmortem rectal lymphoid tissues in Rocky mountain elk (Cervus elaphus nelsoni) infected with chronic wasting disease. jvdi 18: 553-557 [Abstract] [Full Text]
Hamir, A. N., Kunkle, R. A., Cutlip, R. C., Miller, J. M., Williams, E. S., Richt, J. A. (2006). Transmission of chronic wasting disease of mule deer to Suffolk sheep following intracerebral inoculation. jvdi 18: 558-565 [Abstract] [Full Text]
Fox, K. A., Jewell, J. E., Williams, E. S., Miller, M. W. (2006). Patterns of PrPCWD accumulation during the course of chronic wasting disease infection in orally inoculated mule deer (Odocoileus hemionus).. J. Gen. Virol. 87: 3451-3461 [Abstract] [Full Text]
Alverson, J., O'Rourke, K. I., Baszler, T. V. (2006). PrPSc accumulation in fetal cotyledons of scrapie-resistant lambs is influenced by fetus location in the uterus.. J. Gen. Virol. 87: 1035-1041 [Abstract] [Full Text]
Hamir, A. N., Kunkle, R. A., Miller, J. M., Bartz, J. C., Richt, J. A. (2006). First and second cattle passage of transmissible mink encephalopathy by intracerebral inoculation.. Vet Pathol 43: 118-126 [Abstract] [Full Text]
Carmona, P., Monzon, M., Monleon, E., Badiola, J. J., Monreal, J. (2005). In vivo detection of scrapie cases from blood by infrared spectroscopy. J. Gen. Virol. 86: 3425-3431 [Abstract] [Full Text]
Hamir, A. N., Kunkle, R. A., Miller, J. M., Richt, J. A. (2005). Second Passage of Sheep Scrapie and Transmissible Mink Encephalopathy (TME) Agents in Raccoons (Procyon lotor). Vet Pathol 42: 844-851 [Abstract] [Full Text]
Rigter, A., Bossers, A. (2005). Sheep scrapie susceptibility-linked polymorphisms do not modulate the initial binding of cellular to disease-associated prion protein prior to conversion. J. Gen. Virol. 86: 2627-2634 [Abstract] [Full Text]
Williams, E. S. (2005). Chronic Wasting Disease. Vet Pathol 42: 530-549 [Abstract] [Full Text]
Acutis, P. L., Sbaiz, L., Verburg, F., Riina, M. V., Ru, G., Moda, G., Caramelli, M., Bossers, A. (2004). Low frequency of the scrapie resistance-associated allele and presence of lysine-171 allele of the prion protein gene in Italian Biellese ovine breed. J. Gen. Virol. 85: 3165-3172 [Abstract] [Full Text]
Caplazi, P A, O'Rourke, K I, Baszler, T V (2004). Resistance to scrapie in PrP ARR/ARQ heterozygous sheep is not caused by preferential allelic use. J. Clin. Pathol. 57: 647-650 [Abstract] [Full Text]
Casalone, C., Zanusso, G., Acutis, P., Ferrari, S., Capucci, L., Tagliavini, F., Monaco, S., Caramelli, M. (2004). Identification of a second bovine amyloidotic spongiform encephalopathy: Molecular similarities with sporadic Creutzfeldt-Jakob disease. Proc. Natl. Acad. Sci. USA 101: 3065-3070 [Abstract] [Full Text]
Hilton, D A, Sutak, J, Smith, M E F, Penney, M, Conyers, L, Edwards, P, McCardle, L, Ritchie, D, Head, M W, Wiley, C A, Ironside, J W (2004). Specificity of lymphoreticular accumulation of prion protein for variant Creutzfeldt-Jakob disease. J. Clin. Pathol. 57: 300-302 [Abstract] [Full Text]
Monleon, E., Monzon, M., Hortells, P., Vargas, A., Acin, C., Badiola, J. J. (2004). Detection of PrPsc on Lymphoid Tissues from Naturally Affected Scrapie Animals: Comparison of Three Visualization Systems. J. Histochem. Cytochem. 52: 145-151 [Abstract] [Full Text]
Liu, W. G., Brown, D. A., Fraser, J. R. (2003). Immunohistochemical Comparison of Anti-prion Protein (PrP) Antibodies in the CNS of Mice Infected with Scrapie. J. Histochem. Cytochem. 51: 1065-1071 [Abstract] [Full Text]
Hilton, D A, Ironside, J W (2003). Screening for variant Creutzfeldt-Jakob disease. J. Neurol. Neurosurg. Psychiatry 74: 828-829 [Full Text]
Hunter, N. (2003). Scrapie and experimental BSE in sheep. Br Med Bull 66: 171-183 [Abstract] [Full Text]
Herrmann, L. M., Cheevers, W. P., Davis, W. C., Knowles, D. P., O'Rourke, K. I. (2003). CD21-Positive Follicular Dendritic Cells: A Possible Source of PrPSc in Lymph Node Macrophages of Scrapie-Infected Sheep. Am. J. Pathol. 162: 1075-1081 [Abstract] [Full Text]
Hamir, A. N., Miller, J. M., Cutlip, R. C., Stack, M. J., Chaplin, M. J., Jenny, A. L. (2003). Preliminary Observations on the Experimental Transmission of Scrapie to Elk (Cervus elaphus nelsoni) by Intracerebral Inoculation. Vet Pathol 40: 81-85 [Abstract] [Full Text]
Wild, M. A., Spraker, T. R., Sigurdson, C. J., O'Rourke, K. I., Miller, M. W. (2002). Preclinical diagnosis of chronic wasting disease in captive mule deer (Odocoileus hemionus) and white-tailed deer (Odocoileus virginianus) using tonsillar biopsy. J. Gen. Virol. 83: 2629-2634 [Abstract] [Full Text]
Hilton, D. A, Ghani, A. C, Conyers, L., Edwards, P., McCardle, L., Penney, M., Ritchie, D., Ironside, J. W (2002). Accumulation of prion protein in tonsil and appendix: review of tissue samples. BMJ 325: 633-634 [Full Text]
O'Rourke, K. I., Duncan, J. V., Logan, J. R., Anderson, A. K., Norden, D. K., Williams, E. S., Combs, B. A., Stobart, R. H., Moss, G. E., Sutton, D. L. (2002). Active Surveillance for Scrapie by Third Eyelid Biopsy and Genetic Susceptibility Testing of Flocks of Sheep in Wyoming. CVI 9: 966-971 [Abstract] [Full Text]
Tuo, W., O'Rourke, K. I., Zhuang, D., Cheevers, W. P., Spraker, T. R., Knowles, D. P. (2002). Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep. Proc. Natl. Acad. Sci. USA 99: 6310-6315 [Abstract] [Full Text]
Herrmann, L. M., Baszler, T. V., Knowles, D. P., Cheevers, W. P. (2002). PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry. CVI 9: 499-502 [Abstract] [Full Text]
Foster, J. D., Parnham, D. W., Hunter, N., Bruce, M. (2001). Distribution of the prion protein in sheep terminally affected with BSE following experimental oral transmission. J. Gen. Virol. 82: 2319-2326 [Abstract] [Full Text]
Sigurdson, C. J., Spraker, T. R., Miller, M. W., Oesch, B., Hoover, E. A. (2001). PrPCWD in the myenteric plexus, vagosympathetic trunk and endocrine glands of deer with chronic wasting disease. J. Gen. Virol. 82: 2327-2334 [Abstract] [Full Text]
Tuo, W., Zhuang, D., Knowles, D. P., Cheevers, W. P., Sy, M.-S., O'Rourke, K. I. (2001). PrP-C and PrP-Sc at the Fetal-Maternal Interface. J. Biol. Chem. 276: 18229-18234 [Abstract] [Full Text]
Tuo, W., O'Rourke, K. I., Zhuang, D., Cheevers, W. P., Spraker, T. R., Knowles, D. P. (2002). Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep. Proc. Natl. Acad. Sci. USA 99: 6310-6315 [Abstract] [Full Text]

Antimicrob. Agents Chemother.	Clin. Microbiol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS