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Journal of Clinical Microbiology, September 2000, p. 3267-3273, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Variability of Pseudallescheria boydii, a Neurotropic Opportunist

J. Rainer,1,dagger G. S. de Hoog,1,* M. Wedde,2 Y. Gräser,3 and S. Gilges4

Centraalbureau voor Schimmelcultures, Baarn, The Netherlands,1 and Department of Microbiology, University of Technology,2 and Institute of Microbiology and Hygiene, Humboldt University,3 Berlin, and Institute of Hygiene, University of Bonn, Bonn,4 Germany

Received 15 February 2000/Returned for modification 22 March 2000/Accepted 9 June 2000

The sequences of the internal transcribed spacer (ITS) ribosomal DNA (rDNA) domain data obtained by restriction fragment length polymorphism analysis with 18S rDNA and fingerprinting (M13) for clinical and environmental strains of Pseudallescheria boydii (anamorph, Scedosporium apiospermum) were compared to those for related species of Pseudallescheria, Petriella, and Scedosporium. The infraspecific variability of P. boydii was considerable. There were five different lengths in the 18S rDNAs within P. boydii due to the occurrence of introns. In several cases, strains isolated from a single pond or ditch proved to be genetically very different. Nevertheless, some lineages had a regional distribution. The variability found is unlikely to be explained by meiotic recombination alone. Pseudallescheria fusoidea, Pseudallescheria ellipsoidea, and Pseudallescheria angusta were found to be synonyms for P. boydii. Scedosporium prolificans was found amid Petriella species in the ITS tree and showed no infraspecific variability. The type strain of Rhinocladium lesnei proved to be identical to Graphium putredinis. Acladium castellanii, which is morphologically reminiscent of S. apiospermum, was also found to be a separate species, but with an unknown affiliation.

* Corresponding author. Mailing address: Centraalbureau voor Schimmelcultures, P.O. Box 273, NL-3740 AG Baarn, The Netherlands. Phone: 31-35-5481253. Fax: 31-35-5416142. E-mail: de.hoog{at}cbs.knaw.nl.

dagger Present address: Institute of Microbiology (N.F.), Leopold Franzens University, Innsbruck, Austria.

Journal of Clinical Microbiology, September 2000, p. 3267-3273, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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