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Journal of Clinical Microbiology, September 2000, p. 3379-3387, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparative Fingerprinting Analysis of Campylobacter jejuni subsp. jejuni Strains by Amplified-Fragment Length Polymorphism Genotyping

Bjørn-Arne Lindstedt,1,* Even Heir,1 Traute Vardund,1 Kjetil K. Melby,2 and Georg Kapperud1,3

National Institute of Public Health, Department of Bacteriology, N-0403 Oslo,1 Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Sciences, N-0033 Oslo,3 and Department of Microbiology, Ullevaal Hospital, N-0407 Oslo,2 Norway

Received 10 April 2000/Returned for modification 31 May 2000/Accepted 12 July 2000

Amplified-fragment length polymorphism (AFLP) analysis with the endonucleases BglII and MfeI was used to genotype 91 Campylobacter jejuni subsp. jejuni strains from outbreaks and sporadic cases. AFLP-generated fragments were labeled with fluorescent dye and separated by capillary electrophoresis. The software packages GeneScan and GelCompar II were used to calculate AFLP pattern similarities and to investigate phylogenetic relationships among the genotyped strains. The AFLP method was compared with two additional DNA-based typing methods, pulsed-field gel electrophoresis (PFGE) using SmaI and restriction fragment length polymorphism analysis on PCR products (PCR-RFLP) of the flaA and flaB genes. We found that AFLP analysis of C. jejuni strains is a rapid method that offers better discriminatory power than do both PFGE and PCR-RFLP. AFLP and, to a lesser extent, PCR-RFLP could differentiate strains within the same PFGE profiles, which also makes PCR-RFLP an alternative to PFGE. We were able to clearly distinguish 9 of 10 recognized outbreaks by AFLP and to identify similarities among outbreak and sporadic strains. Therefore, AFLP is suitable for epidemiological surveillance of C. jejuni and will be an excellent tool for source identification in outbreak situations.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Public Health, Geitmyrsveien 75, P.O. Box 4404 Torshov, N-0403 Oslo, Norway. Phone: 47 22042200. Fax: 47 22042518. E-mail: bjorn-arne.lindstedt{at}folkehelsa.no.

Journal of Clinical Microbiology, September 2000, p. 3379-3387, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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