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Journal of Clinical Microbiology, September 2000, p. 3415-3419, Vol. 38, No. 9
Laboratoire des Microbiologie-Immunologie,
Hopital Universitaire, Farhat Hached, Sousse,
Tunisia,1 and MRC/MEDUNSA Diarrhoeal
Pathogens Research Unit, Medical University of Southern Africa,
Pretoria, South Africa2
Received 22 November 1999/Returned for modification 18 February
2000/Accepted 26 June 2000
Rotavirus strains circulating in Sousse, Tunisia, between 1995 and
1999 were characterized antigenically by monoclonal antibodies to the
VP6 subgroup and the VP7 serotype. The VP4 genotype was determined by
reverse transcription-PCR, as were the strains with untyped VP7. Only
17% of 375 children were shedding rotavirus as determined by latex
agglutination assay. Most rotavirus strains were G1P[8] (50%),
followed by G4P[8] and G4P[6]. Reassortant G1P[4] strains emerged
in Sousse during the 1998-1999 season.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Distribution of Rotavirus VP7 Serotypes and VP4
Genotypes Circulating in Sousse, Tunisia, from 1995 to 1999:
Emergence of Natural Human Reassortants
*
Corresponding author. Mailing address: MRC/MEDUNSA
Diarrhoeal Pathogens Research Unit, P. O. Box 173, MEDUNSA 0204, Pretoria, South Africa. Phone: (27 12) 521 5938. Fax: (27 12) 521 5794. E-mail: dsteele{at}virol1.medunsa.ac.za.
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