Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects

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Journal of Clinical Microbiology, September 2000, p. 3436-3441, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects

Ann-Catrin E. Thoreson,¹ Annika Hamlet,¹^, Janet Çelik,² Mona Byström,³ Susanne Nyström,³ Lars Olbe,⁴ and Ann-Mari Svennerholm¹^,^*

Departments of Medical Microbiology and Immunology¹ and Surgery,⁴ Göteborg University, S-413 46 Göteborg, Microbiology and Tumor-Biology Center, Karolinska Institute, S-171 77 Stockholm,² and AstraZeneca, Umeå,³ Sweden

Received 20 December 1999/Returned for modification 11 February 2000/Accepted 2 June 2000

We have analyzed possible qualitative and quantitative differences in antigen expression between Helicobacter pylori strainsisolated from the antrum and different locations in the duodenumof 21 duodenal ulcer (DU) patients and 20 asymptomatic subjects(AS) by enzyme-linked immunosorbent assay (ELISA) and inhibitionELISA. Almost all antral and duodenal strains grown in vitro expressedthe N-acetyl-neuroaminyllactose-binding hemagglutinin, flagellins(subunits FlaA and FlaB), urease, a 26-kDa protein, and a neutrophil-activatingprotein. In 75% of both the DU patients and the AS, antral H.pylori strains expressed either the blood group antigen Lewisy (Le^y) alone or together with the Le^x antigen. However, duodenal H. pylori strains of DU patients expressedLe^y antigen more frequently than corresponding strains of AS (P <0.05). Presence of Le^y on H. pylori was related to the degree of active duodenitis (P< 0.05). Duodenal H. pylori strains isolated from AS were significantlymore often Lewis nontypeable than duodenal strains of DU patients(P < 0.01). Presence of H. pylori blood group antigen-bindingadhesin (BabA) was significantly higher on both antral and duodenalstrains isolated from DU patients than on corresponding strainsisolated from AS (P < 0.05). BabA-positive duodenal H. pyloristrains isolated from DU patients were associated with activeduodenitis more frequently than corresponding strains isolatedfrom AS (P < 0.01). Infection with H. pylori strains positivefor Le^y and BabA in the duodenum is associated with development of duodenalulcerformation.

^* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Guldhedsgatan 10 A, S-413 46 Göteborg,Sweden. Phone: 46-31-342 47 24. Fax: 46-31-82 69 76. E-mail: annmari.svennerholm{at}microbio.gu.se.

Present address: The Department of Medicine, Intestinal Disease Research Program, McMaster University Medical Center, Hamilton,Ontario,Canada.

Journal of Clinical Microbiology, September 2000, p. 3436-3441, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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