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Journal of Clinical Microbiology, September 2000, p. 3436-3441, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects

Ann-Catrin E. Thoreson,1 Annika Hamlet,1,dagger Janet Çelik,2 Mona Byström,3 Susanne Nyström,3 Lars Olbe,4 and Ann-Mari Svennerholm1,*

Departments of Medical Microbiology and Immunology1 and Surgery,4 Göteborg University, S-413 46 Göteborg, Microbiology and Tumor-Biology Center, Karolinska Institute, S-171 77 Stockholm,2 and AstraZeneca, Umeå,3 Sweden

Received 20 December 1999/Returned for modification 11 February 2000/Accepted 2 June 2000

We have analyzed possible qualitative and quantitative differences in antigen expression between Helicobacter pylori strains isolated from the antrum and different locations in the duodenum of 21 duodenal ulcer (DU) patients and 20 asymptomatic subjects (AS) by enzyme-linked immunosorbent assay (ELISA) and inhibition ELISA. Almost all antral and duodenal strains grown in vitro expressed the N-acetyl-neuroaminyllactose-binding hemagglutinin, flagellins (subunits FlaA and FlaB), urease, a 26-kDa protein, and a neutrophil-activating protein. In 75% of both the DU patients and the AS, antral H. pylori strains expressed either the blood group antigen Lewis y (Ley) alone or together with the Lex antigen. However, duodenal H. pylori strains of DU patients expressed Ley antigen more frequently than corresponding strains of AS (P < 0.05). Presence of Ley on H. pylori was related to the degree of active duodenitis (P < 0.05). Duodenal H. pylori strains isolated from AS were significantly more often Lewis nontypeable than duodenal strains of DU patients (P < 0.01). Presence of H. pylori blood group antigen-binding adhesin (BabA) was significantly higher on both antral and duodenal strains isolated from DU patients than on corresponding strains isolated from AS (P < 0.05). BabA-positive duodenal H. pylori strains isolated from DU patients were associated with active duodenitis more frequently than corresponding strains isolated from AS (P < 0.01). Infection with H. pylori strains positive for Ley and BabA in the duodenum is associated with development of duodenal ulcer formation.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Guldhedsgatan 10 A, S-413 46 Göteborg, Sweden. Phone: 46-31-342 47 24. Fax: 46-31-82 69 76. E-mail: annmari.svennerholm{at}microbio.gu.se.

dagger Present address: The Department of Medicine, Intestinal Disease Research Program, McMaster University Medical Center, Hamilton, Ontario, Canada.

Journal of Clinical Microbiology, September 2000, p. 3436-3441, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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