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Journal of Clinical Microbiology, September 2000, p. 3453-3456, Vol. 38, No. 9
0095-1137/00/$04.00+0

Use of Coagulase Gene (coa) Repeat Region Nucleotide Sequences for Typing of Methicillin-Resistant Staphylococcus aureus Strains

B. Shopsin,1,2 M. Gomez,1 M. Waddington,3 M. Riehman,3 and B. N. Kreiswirth1,*

Public Health Research Institute1 and Department of Microbiology, New York University School of Medicine,2 New York, New York 10016, and MIDI Labs, Inc., Newark, Delaware 197133

Received 24 January 2000/Returned for modification 4 May 2000/Accepted 13 June 2000

Coagulase gene (coa) short sequence repeat region sequencing was used to measure relatedness among a collection of temporally and geographically diverse methicillin-resistant Staphylococcus aureus isolates. The results show that coa polymorphism is free of strong selective pressure and has a low index of variation that may be useful for long-term epidemiological investigations. coa typing is a useful addition to spa typing for analysis of S. aureus, including methicillin-resistant strains.


* Corresponding author. Mailing address: PHRI TB Center, 455 First Ave., New York, NY 10016. Phone: (212) 578-0850. Fax: (212) 578-0853. E-mail: barry{at}phri.org.


Journal of Clinical Microbiology, September 2000, p. 3453-3456, Vol. 38, No. 9
0095-1137/00/$04.00+0



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