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Journal of Clinical Microbiology, September 2000, p. 3470-3473, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:Hminus Strains and a Source of Human Diseases

Martina Bielaszewska,1,2,* Herbert Schmidt,2 Almut Liesegang,3 Rita Prager,3 Wolfgang Rabsch,3 Helmut Tschäpe,3 Alois Cízek,4 Jan Janda,5 Kveta Bláhová,5 and Helge Karch2

Institute for Medical Microbiology, The 2nd Medical Faculty, Charles University,1 and 1st Clinic of Pediatrics, University Hospital Motol,5 150 06 Prague, and Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, 602 00 Brno,4 Czech Republic, and Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg,2 and Robert Koch Institut, Bereich Wernigerode, 38855 Wernigerode,3 Germany

Received 21 April 2000/Returned for modification 27 May 2000/Accepted 13 June 2000

Using the immunomagnetic separation procedure, we isolated sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H- strains from two patients, one with hemolytic-uremic syndrome and the other with diarrhea, and from a dairy cow epidemiologically associated with the patients. The phenotypic and genotypic characteristics of all isolates were identical or closely related. Moreover, the bovine isolate showed a clonal relatedness to SF STEC O157:H- strains isolated from patients in Germany and the Czech Republic from 1988 to 1998. This is the first evidence that cattle can be a reservoir of SF STEC O157:H- and a source of human diseases.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany. Phone: 49-931/201 51 63. Fax: 49-931/201 51 66. E-mail: mbielaszewska{at}hygiene.uni-wuerzburg.de.

Journal of Clinical Microbiology, September 2000, p. 3470-3473, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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