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Journal of Clinical Microbiology, September 2000, p. 3474-3477, Vol. 38, No. 9
Veterinary Infectious Disease Organization,
University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E3
Received 24 February 2000/Returned for modification 3 May
2000/Accepted 22 June 2000
A competitive PCR (cPCR) assay was developed for monitoring porcine
circovirus (PCV) DNA in serum samples from piglets. The cPCR was based
on competitive coamplification of a 502- or 506-bp region of the PCV
type 1 (PCV1) or PCV2 ORF2, respectively, with a known concentration of
competitor DNA, which produced a 761- or 765-bp fragment, respectively.
The cPCR was validated by quantification of a known amount of PCV
wild-type plasmids. We also used this technique to determine PCV genome
copy numbers in infected cells. Furthermore, we measured PCV DNA loads
in clinical samples. More than 50% of clinically healthy piglets could
harbor both types of PCV. While PCV1 was detected in only 3 of 16 pigs
with postweaning multisystemic wasting syndrome (PMWS), all the sick
piglets contained PCV2. A comparison of the PCV2 DNA loads of healthy
and sick animals revealed a significant difference, indicating that the
development of PMWS may require a certain amount of PCV2.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Quantitative, Competitive PCR Analysis of Porcine
Circovirus DNA in Serum from Pigs with Postweaning Multisystemic
Wasting Syndrome

*
Corresponding author. Mailing address: VIDO, 120 Veterinary Rd., Saskatoon, Saskatchewan, Canada S7N 5E3. Phone:
1-306-966 7483. Fax: 1-306-966 7478. E-mail:
liuq{at}sask.usask.ca.
Present address: American Animal Health, Inc., Grand Prairie,
TX 75052.
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