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Journal of Clinical Microbiology, September 2000, p. 3478-3480, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR for Diagnosis of Paracoccidioidomycosis

Glauce M. Gomes,1 Patrícia S. Cisalpino,2 Carlos P. Taborda,1 and Zoilo P. de Camargo1,*

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP,1 and Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG,2 Brazil

Received 7 January 2000/Returned for modification 11 March 2000/Accepted 27 June 2000

A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.

* Corresponding author. Mailing address: Disciplina de Biologia Celular, Universidade Federal de São Paulo, 04023-062, Rua Botucatu 862/8° Andar, São Paulo, SP, Brazil. Phone: 55 11-576 45 23. Fax: 55 11-571 58 77. E-mail: zoilo{at}ecb.epm.br.

Journal of Clinical Microbiology, September 2000, p. 3478-3480, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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