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Journal of Clinical Microbiology, September 2000, p. 3484-3488, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of DT104 and U302 Phage Types among Salmonella enterica Serotype Typhimurium Isolates by PCR

Lori C. Pritchett,1,* Michael E. Konkel,2 John M. Gay,1 and Thomas E. Besser3

Field Disease Investigation Unit, College of Veterinary Medicine,1 Department of Microbiology, College of Sciences,2 and Department of Veterinary Microbiology and Pathology,3 College of Veterinary Medicine, Washington State University, Pullman, Washington 99164

Received 2 March 2000/Returned for modification 24 April 2000/Accepted 21 June 2000

A DNA sequence was identified in isolates of Salmonella enterica serotype Typhimurium definitive type 104 (DT104). The PCR amplification of an internal segment of this sequence identified DT104 and the closely related U302 phage type among 146 isolates of S. enterica serotype Typhimurium tested, thus providing a tool for rapid identification of DT104 and related isolates.

* Corresponding author. Mailing address: Field Disease Investigation Unit, College of Veterinary Medicine, Washington State University, Pullman, WA 99164. Phone: (509) 335-0808. Fax: (509) 335-0880. E-mail: lcp{at}vetmed.wsu.edu.

Journal of Clinical Microbiology, September 2000, p. 3484-3488, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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