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Journal of Clinical Microbiology, September 2000, p. 3492-3494, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Molecular Cloning, Expression, and Antigenicity of
Seto Virus Belonging to Genogroup I Norwalk-Like Viruses
Shinichi
Kobayashi,1,*
Kenji
Sakae,1
Yasumoto
Suzuki,1
Kuniko
Shinozaki,2
Mineyuki
Okada,2
Hiroaki
Ishiko,3
Kunio
Kamata,4
Kenji
Suzuki,5
Katsuro
Natori,6
Tatsuo
Miyamura,6 and
Naokazu
Takeda6
Laboratory of Virology, Aichi Prefectural
Institute of Public Health, Tujimachi, Kita-ku, Nagoya
462-8576,1 Laboratory of Virology,
Public Health Laboratory of Chiba Prefecture, Chuoh-ku, Chiba
260-8715,2 Infectious Diseases Test
Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories
Inc., Itabashi-ku, Tokyo 174-8555,3
Viral Diagnostics Production Department, Denka-seiken Co.,
Ltd., Gosen, Niigata 959-1695,4 and
Department of Biochemistry and Cell
Biology5 and Department of Virology
II,6 National Institute of Infectious Diseases,
Sinjuku-ku, Tokyo 162-8640, Japan
Received 7 January 2000/Returned for modification 13 April
2000/Accepted 30 June 2000
The viral capsid protein of the Seto virus (SeV), a Japanese strain
of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like
particles using a baculovirus expression system. An antigen detection
enzyme-linked immunosorbent assay based on hyperimmune antisera to
recombinant SeV was highly specific to homologous SeV-like strains but
not heterologous strains in stools, allowing us type-specific detection
of NLVs.
*
Corresponding author. Mailing address: Laboratory of
Virology, Aichi Prefectural Institute of Public Health, 7-6 Nagare,
Tujimachi, Kita-ku, Nagoya 462-8576, Japan. Phone: (81)-52-911-3111.
Fax: (81)-52-913-3641. E-mail:
shinkoba{at}he.mirai.ne.jp.
Journal of Clinical Microbiology, September 2000, p. 3492-3494, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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