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Journal of Clinical Microbiology, September 2000, p. 3492-3494, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses

Shinichi Kobayashi,1,* Kenji Sakae,1 Yasumoto Suzuki,1 Kuniko Shinozaki,2 Mineyuki Okada,2 Hiroaki Ishiko,3 Kunio Kamata,4 Kenji Suzuki,5 Katsuro Natori,6 Tatsuo Miyamura,6 and Naokazu Takeda6

Laboratory of Virology, Aichi Prefectural Institute of Public Health, Tujimachi, Kita-ku, Nagoya 462-8576,1 Laboratory of Virology, Public Health Laboratory of Chiba Prefecture, Chuoh-ku, Chiba 260-8715,2 Infectious Diseases Test Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories Inc., Itabashi-ku, Tokyo 174-8555,3 Viral Diagnostics Production Department, Denka-seiken Co., Ltd., Gosen, Niigata 959-1695,4 and Department of Biochemistry and Cell Biology5 and Department of Virology II,6 National Institute of Infectious Diseases, Sinjuku-ku, Tokyo 162-8640, Japan

Received 7 January 2000/Returned for modification 13 April 2000/Accepted 30 June 2000

The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.

* Corresponding author. Mailing address: Laboratory of Virology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tujimachi, Kita-ku, Nagoya 462-8576, Japan. Phone: (81)-52-911-3111. Fax: (81)-52-913-3641. E-mail: shinkoba{at}he.mirai.ne.jp.

Journal of Clinical Microbiology, September 2000, p. 3492-3494, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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