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Journal of Clinical Microbiology, September 2000, p. 3505-3507, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pseudo-Outbreak of Imipenem-Resistant Acinetobacter baumannii Resulting from False Susceptibility Testing by a Rapid Automated System

Athanassios Tsakris,1,* Anastassia Pantazi,2 Spyros Pournaras,3 Antonios Maniatis,3 Aleka Polyzou,2 and Danai Sofianou2

Department of Microbiology, Medical School, Aristotle University of Thessaloniki,1 Department of Microbiology, Hippokration General Hospital,2 Thessaloniki, and Department of Medical Microbiology, University of Thessalia,3 Greece

Received 15 March 2000/Returned for modification 3 May 2000/Accepted 27 June 2000

Introduction of the Vitek GNS-506 susceptibility testing cards in the Hippokration General Hospital, Thessaloniki, Greece, resulted in an apparently high prevalence of imipenem-resistant Acinetobacter baumannii. When 35 of these isolates were further tested by disk diffusion, broth microdilution, and agar dilution assays, 32 were imipenem sensitive by all tests and three were sensitive or intermediate, depending on the method. The pseudoresistant acinetobacters did not form a genetically homogeneous group. It is suggested that the detection of imipenem-resistant A. baumannii isolates by this system should be confirmed by an additional susceptibility test.

* Corresponding author. Mailing address: Department of Microbiology, Medical School, Aristotle University of Thessaloniki, 54 006 Thessaloniki, Greece. Phone: 30 31 99 90 91. Fax: 30 31 99 91 49. E-mail: atsakris{at}med.auth.gr.

Journal of Clinical Microbiology, September 2000, p. 3505-3507, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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