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Journal of Clinical Microbiology, September 2000, p. 3508-3510, Vol. 38, No. 9
0095-1137/00/$04.00+0

Comparison of Rapid Centrifugation Assay with Conventional Tissue Culture Method for Isolation of Dengue 2 Virus in C6/36-HT Cells

Rosmari Rodríguez Roche, Mayling Alvarez, María G. Guzmán,* Luis Morier, and Gustavo Kourí

Department of Virology, PAHO/WHO Collaborator Center for Viral Diseases, "Pedro Kourí" Tropical Medicine Institute, Havana, Cuba

Received 2 December 1999/Returned for modification 7 February 2000/Accepted 11 June 2000

A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.

* Corresponding author. Mailing address: Department of Virology, "Pedro Kourí" Tropical Medicine Institute (IPK), Autopista Novia del Mediodía y Carretera Central Km 61/2, La Lisa, P.O. Box 601 Marianao 13, Havana, Cuba. Phone: 53-7-220450. Fax: 53-7-246051. E-mail: lupe{at}ipk.sld.cu.

Journal of Clinical Microbiology, September 2000, p. 3508-3510, Vol. 38, No. 9
0095-1137/00/$04.00+0


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