Transmission Dynamics and Molecular Characterization of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110

doi:10.1128/JCM.39.1.217-221.2001

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Journal of Clinical Microbiology, January 2001, p. 217-221, Vol. 39, No. 1
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.1.217-221.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transmission Dynamics and Molecular Characterization of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110

Hanna Soini,¹ Xi Pan,¹ Larry Teeter,¹ James M. Musser,² and Edward A. Graviss¹^,³^,^*

Departments of Pathology¹ and Medicine,³ Baylor College of Medicine, Houston, Texas 77030, and Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840²

Received 22 August 2000/Returned for modification 19 September 2000/Accepted 10 October 2000

Population-based analysis of Mycobacterium tuberculosis transmission in Houston, Tex., over 5 years identified 377 patientsinfected with an isolate containing one to four copies of IS6110.The isolates were analyzed by spoligotyping and assigned to oneof three major genetic groups based on nucleotide polymorphismsin codons katG 463 and gyrA 95. Prospectively obtained patientinterviews were reviewed to assess epidemiologic links betweenapparently clustered patients. A total of 13 groups of isolateswith the same IS6110 profile were identified, representing 326of the 377 patients (86.5%; range 2 to 113 patients). In contrast,28 groups of isolates containing 334 patients (88.6%) had thesame spoligotype (range, 2 to 143 patients). Combination of IS6110profile and spoligotype data identified 31 clusters with 300 patients(79.6%; range, 2 to 82 patients). All 377 isolates belonged tomajor genetic group 1 (77 patients) or genetic group 2 (300 patients);no major genetic group 3 isolates were identified. Among the 228patients interviewed, 33 patients (14.5%) were directly linkedto another patient in the same cluster. Possible epidemiologiclinks were also found among 11 patients. Moreover, many clustersconsisted of individuals with the same ethnicity. In conclusion,we confirmed that IS6110 profiling and spoligotyping togetherprovide enhanced molecular discrimination of M. tuberculosis isolateswith low copy numbers of IS6110. Identification of epidemiologiclinks among some of the patients verified that the combinationof these two methods reliably indexes tuberculosistransmission.

^* Corresponding author. Mailing address: Department of Pathology (209E), Baylor College of Medicine, One Baylor Plaza, Houston,TX 77030-3498. Phone: (713) 798-8097. Fax: (713) 798-8895. E-mail:egraviss{at}bcm.tmc.edu.

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