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Journal of Clinical Microbiology, January 2001, p. 222-227, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.222-227.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Typing of Mycobacterium bovis Isolates from Cameroon

Berthe M. Njanpop-Lafourcade,1 Jacqueline Inwald,2 Annick Ostyn,1 Benoît Durand,3 Steven Hughes,2 Marie-Françoise Thorel,1 Glyn Hewinson,2 and Nadia Haddad1,*

Secteur des Mycobactéries, Unité des Zoonoses Bactériennes,1 and Unité d'Epidémiologie,3 Agence Française de Sécurité Sanitaire des Aliments Alfort, Maisons-Alfort, France, and TB Research Group, Bacteriology Department, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, England2

Received 3 January 2000/Returned for modification 26 March 2000/Accepted 23 August 2000

In order to gain a better understanding of the molecular epidemiology of Mycobacterium bovis isolates in Cameroon, 75 isolates of M. bovis collected in three provinces of northern Cameroon were studied by spoligotyping. For 65 of these isolates, typing was also carried out by pulsed-field gel electrophoresis (PFGE) with DraI, and 18 of the isolates were also typed by restriction fragment length polymorphism (RFLP) analysis with probe IS6110-RHS. Molecular typing of the isolates by these techniques revealed a high degree of homogeneity, with 10 spoligotypes for 75 isolates, four PFGE profiles for 65 isolates, and three RFLP types for 18 isolates. Some types were present in the three different provinces, while some were confined to one or two areas. These results suggest that geographical mapping of M. bovis strains could be helpful for the control of bovine tuberculosis at the regional level. An interesting feature of all the spoligotypes was the absence of spacer 30, suggesting a common origin for all of the Cameroon isolates tested; an evolutionary scenario for the isolates is discussed. In addition, a comparison of the three techniques showed that for M. bovis strain differentiation in Cameroon and in surrounding countries, spoligotyping would be a more discriminating and practical tool for molecular typing than the other two techniques used in this study.

* Corresponding author. Present address: Ecole Nationale Vétérinaire de Maisons Alfort, UP de Maladies Contagieuses, 7 Ave. du Général de Gaulle, F-94704 Maisons-Alfort Cedex, France. Phone: 33/1 43 96 71 32. Fax: 33/1 43 96 71 31. E-mail: haddad{at}vet-alfort.fr.

Journal of Clinical Microbiology, January 2001, p. 222-227, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.222-227.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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