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Journal of Clinical Microbiology, January 2001, p. 323-327, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.323-327.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Differentiation of Candida dubliniensis from Candida albicans on Staib Agar and Caffeic Acid-Ferric Citrate Agar

Asmaa Al Mosaid,1 Derek Sullivan,1 Ira F. Salkin,2 Diarmuid Shanley,1 and David C. Coleman1,*

Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland,1 and Wadsworth Center, New York State Department of Health, Albany, New York2

Received 20 July 2000/Returned for modification 23 September 2000/Accepted 21 October 2000

The methods currently available for the identification of the pathogenic yeast Candida dubliniensis all have disadvantages in that they are time-consuming, expensive, and/or, in some cases, unreliable. In a recent study (P. Staib and J. Morschhäuser, Mycoses 42:521-524; 1999) of 14 C. dubliniensis and 11 C. albicans isolates, it was suggested that the ability of C. dubliniensis to produce rough colonies and chlamydospores (chlamydoconidia) on Staib agar (SA) provided a simple means of differentiating it from its close relative C. albicans. In the present investigation, we examined the colony morphology and chlamydospore production of 130 C. dubliniensis and 166 C. albicans isolates on SA and on the related defined medium caffeic acid-ferric citrate agar (CAF). All of the C. dubliniensis and C. albicans isolates produced chlamydospores on the control medium, i.e., rice-agar-Tween agar. However, while none of the C. albicans isolates produced chlamydospores on either SA or CAF, 85.4 and 83.8% of the C. dubliniensis isolates produced chlamydospores on SA and CAF, respectively. All of the C. albicans isolates grew as smooth, shiny colonies on SA after 48 to 72 h of incubation at 30°C, while 97.7% of the C. dubliniensis isolates grew as rough colonies, many (65%) with a hyphal fringe. In contrast, 87.4% of the C. albicans and 93.8% of the C. dubliniensis isolates yielded rough colonies on CAF. Although the results of this study confirm that SA is a good medium for distinguishing between C. dubliniensis and C. albicans, we believe that discrimination between these two species is best achieved on the basis of colony morphology rather than chlamydospore production.

* Corresponding author. Mailing address: University of Dublin, Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science, Trinity College, Dublin 2, Republic of Ireland. Phone: 353 1 6127276. Fax: 353 1 6127295. E-mail: dcoleman{at}dental.tcd.ie.

Journal of Clinical Microbiology, January 2001, p. 323-327, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.323-327.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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