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Journal of Clinical Microbiology, January 2001, p. 328-331, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.328-331.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Binary Typing of Staphylococcus aureus Strains through Reversed Hybridization Using Digoxigenin-Universal Linkage System-Labeled Bacterial Genomic DNA

Willem van Leeuwen,1,* Christel Libregts,2 Marjolijn Schalk,2 Jack Veuskens,2 Henri Verbrugh,1 and Alex van Belkum1

Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GD Rotterdam,1 and KREATECH Diagnostics, 1032 LG Amsterdam,2 The Netherlands

Received 17 July 2000/Returned for modification 18 September 2000/Accepted 12 October 2000

A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.

* Corresponding author. Mailing address: Erasmus University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31-10-4633668. Fax: 31-10-4633875. E-mail: vanleeuwen{at}bacl.azr.nl.

Journal of Clinical Microbiology, January 2001, p. 328-331, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.328-331.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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