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Journal of Clinical Microbiology, January 2001, p. 332-334, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.332-334.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of the Triage Micro Parasite Panel for Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum in Patient Stool Specimens

Susan E. Sharp,^* Clarisa A. Suarez, Yolanda Duran, and Robert J. Poppiti

Department of Pathology and Laboratory Medicine, Mount Sinai Medical Center, Miami Beach, Florida 33140

Received 7 August 2000/Returned for modification 11 September 2000/Accepted 4 October 2000

A study comparing the Triage Micro Parasite Panel (Biosite Diagnostics, Inc., San Diego, Calif.) to conventional O&P examination (O&P) was performed using patient fecal specimens. Five hundred twenty-three stool samples were compared. Nineteen specimens were found to be positive by Triage, and 29 were found to be positive by O&P. Seven specimens were positive for Giardia lamblia, four were positive for Entamoeba histolytica/E. dispar, and three were positive for Cryptosporidium parvum as determined by both methods. There was one false positive by Triage (C. parvum) and four false negatives by O&P (two G. lamblia, one E. histolytica/E. dispar, and one C. parvum). The Triage test accurately detected all 18 specimens that contained one of the three organisms that it was designed to detect. The Triage test is a rapid, easy-to-use enzyme immunoassay for the detection of G. lamblia, E. histolytica/E. dispar, and C. parvum in fresh or fresh-frozen fecal specimens. These data suggest that the Triage test can be used as a screen for the immediate testing of stool specimens for these three pathogenic parasites. If Triage test results are negative, O&P can be performed if parasitic infections other than G. lamblia, E. histolytica/E. dispar, or C. parvum are suspected.

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^* Corresponding author. Mailing address: Mount Sinai Medical Center, Department of Pathology and Laboratory Medicine, 4300 Alton Rd., Miami Beach, FL 33140. Phone: (305) 674-2265. Fax: (305) 535-7938. E-mail: sue-sharp{at}msmc.com.

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Journal of Clinical Microbiology, January 2001, p. 332-334, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.332-334.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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