This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ravyn, M. D.
Right arrow Articles by Johnson, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ravyn, M. D.
Right arrow Articles by Johnson, R. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2001, p. 335-338, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.335-338.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation of the Etiologic Agent of Human Granulocytic Ehrlichiosis from the White-Footed Mouse (Peromyscus leucopus)

M. Dana Ravyn,1 Carrie B. Kodner,1 Sarah E. Carter,1 Janet L. Jarnefeld,2 and Russell C. Johnson1,*

Department of Microbiology, University of Minnesota Academic Health Center, Minneapolis, Minnesota 55455,1 and Metropolitan Mosquito Control District, St. Paul, Minnesota 551042

Received 12 April 2000/Returned for modification 5 July 2000/Accepted 18 October 2000

We examined white-footed mice (Peromyscus leucopus) from Minnesota for infection with the etiologic agent of human granulocytic ehrlichiosis (HGE). From April to September 1997, we collected P. leucopus from Washington County, Minnesota, an area enzootic for HGE. Blood was cultivated in HL60 cells for isolation of the HGE agent. Of 59 mice examined, only a single mouse was culture positive for the HGE agent. The 16S ribosomal DNA sequence of the isolate was determined to be identical to that of the HGE agent. The isolate was reactive with monoclonal antibodies to the 44-kDa antigen of the HGE agent and was infectious for laboratory mice.

* Corresponding author. Mailing address: University of Minnesota Academic Health Center, 420 Delaware St., S.E., Minneapolis, MN 55455-0312. Phone: (612) 624-5684. Fax: (612) 626-0623. E-mail: johnson{at}mail.ahc.umn.edu.

Journal of Clinical Microbiology, January 2001, p. 335-338, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.335-338.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Felek, S., Telford, S. III, Falco, R. C., Rikihisa, Y. (2004). Sequence Analysis of p44 Homologs Expressed by Anaplasma phagocytophilum in Infected Ticks Feeding on Naive Hosts and in Mice Infected by Tick Attachment. Infect. Immun. 72: 659-666 [Abstract] [Full Text]  
  • Carlyon, J. A., Akkoyunlu, M., Xia, L., Yago, T., Wang, T., Cummings, R. D., McEver, R. P., Fikrig, E. (2003). Murine neutrophils require {alpha}1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. Blood 102: 3387-3395 [Abstract] [Full Text]  
  • Yago, T., Leppanen, A., Carlyon, J. A., Akkoyunlu, M., Karmakar, S., Fikrig, E., Cummings, R. D., McEver, R. P. (2003). Structurally Distinct Requirements for Binding of P-selectin Glycoprotein Ligand-1 and Sialyl Lewis x to Anaplasma phagocytophilum and P-selectin. J. Biol. Chem. 278: 37987-37997 [Abstract] [Full Text]  
  • Carter, S. E., Ravyn, M. D., Xu, Y., Johnson, R. C. (2001). Molecular Typing of the Etiologic Agent of Human Granulocytic Ehrlichiosis. J. Clin. Microbiol. 39: 3398-3401 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»