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Journal of Clinical Microbiology, January 2001, p. 343-346, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.343-346.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Single-Tube Single-Enzyme Reverse Transcriptase PCR Assay for Detection of Bovine Viral Diarrhea Virus in Pooled Bovine Serum

Daniel Weinstock,* Bodreddigari Bhudevi, and Anthony E. Castro

Animal Diagnostic Laboratory, Department of Veterinary Science, Pennsylvania State University, University Park, Pennsylvania 16802

Received 10 April 2000/Returned for modification 25 June 2000/Accepted 24 September 2000

A reverse transcriptase PCR (RT-PCR) was developed for use as a diagnostic screening test for the detection of bovine viral diarrhea virus (BVDV) in pooled bovine serum samples. Individual serum samples from 60 dairy cattle herds located in Pennsylvania were evaluated by the microplate virus isolation method, and pooled sera were analyzed by RT-PCR. RT-PCR was sensitive and specific and detected a single viremic serum sample in up to 100 pooled serum samples. RT-PCR analysis of pooled sera provides a rapid and cost-effective method for the screening of cattle herds for the presence of animals persistently infected with BVDV.

* Corresponding author. Mailing address: Animal Diagnostic Laboratory, Orchard Road, Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-1949. Fax: (814) 865-3907. E-mail: dxw31{at}psu.edu.

Journal of Clinical Microbiology, January 2001, p. 343-346, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.343-346.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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