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Journal of Clinical Microbiology, January 2001, p. 347-350, Vol. 39, No. 1
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.1.347-350.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Comparative Prevalences of Brachyspira
aalborgi and Brachyspira (Serpulina)
pilosicoli as Etiologic Agents of Histologically Identified
Intestinal Spirochetosis in Australia
Andrew S. J.
Mikosza,1
Tom
La,1
W. Bastiaan
de
Boer,2 and
David J.
Hampson1,*
Division of Veterinary and Biomedical
Sciences, Murdoch University, Murdoch, Western Australia
6150,1 and Department of Anatomical
Pathology, PathCentre, Nedlands, Western Australia
6009,2 Australia
Received 20 April 2000/Returned for modification 29 September
2000/Accepted 19 October 2000
DNA from gastrointestinal biopsy specimens from 28 Australian
patients with histologic evidence of intestinal spirochetosis (IS) was
subjected to PCRs to amplify segments of the 16S rRNA and NADH oxidase
genes of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli. B. aalborgi was identified in
specimens from 24 (85.7%) patients and B. pilosicoli in
those from 4 (14.3%) patients (2 of whom were also positive for
B. aalborgi). For two patients, no product was amplified.
This study demonstrates that B. aalborgi is much more
commonly involved in histologically identified IS in Australian
patients than is B. pilosicoli. This is the first report of
amplification of B. pilosicoli DNA from humans with IS.
*
Corresponding author. Mailing address: Division of
Veterinary and Biomedical Sciences, Murdoch University, Murdoch,
Western Australia 6150, Australia. Phone: 61 08 9360 2287. Fax: 61 08 9310 4144. E-mail: hampson{at}numbat.murdoch.edu.au.
Journal of Clinical Microbiology, January 2001, p. 347-350, Vol. 39, No. 1
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.1.347-350.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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