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Journal of Clinical Microbiology, January 2001, p. 347-350, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.347-350.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparative Prevalences of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as Etiologic Agents of Histologically Identified Intestinal Spirochetosis in Australia

Andrew S. J. Mikosza,1 Tom La,1 W. Bastiaan de Boer,2 and David J. Hampson1,*

Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150,1 and Department of Anatomical Pathology, PathCentre, Nedlands, Western Australia 6009,2 Australia

Received 20 April 2000/Returned for modification 29 September 2000/Accepted 19 October 2000

DNA from gastrointestinal biopsy specimens from 28 Australian patients with histologic evidence of intestinal spirochetosis (IS) was subjected to PCRs to amplify segments of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli. B. aalborgi was identified in specimens from 24 (85.7%) patients and B. pilosicoli in those from 4 (14.3%) patients (2 of whom were also positive for B. aalborgi). For two patients, no product was amplified. This study demonstrates that B. aalborgi is much more commonly involved in histologically identified IS in Australian patients than is B. pilosicoli. This is the first report of amplification of B. pilosicoli DNA from humans with IS.


* Corresponding author. Mailing address: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia. Phone: 61 08 9360 2287. Fax: 61 08 9310 4144. E-mail: hampson{at}numbat.murdoch.edu.au.


Journal of Clinical Microbiology, January 2001, p. 347-350, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.347-350.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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