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Journal of Clinical Microbiology, January 2001, p. 351-353, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.351-353.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Accuracy of the VITEK 2 System To Detect Glycopeptide Resistance in Enterococci

Nicole van den Braak,* Wil Goessens, Alex van Belkum, Henri A. Verbrugh, and Hubert P. Endtz

Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands

Received 19 June 2000/Returned for modification 10 August 2000/Accepted 5 October 2000

We evaluated the accuracy of the VITEK 2 fully automated system to detect and identify glycopeptide-resistant enterococci (GRE) compared to a reference agar dilution method. The sensitivity of vancomycin susceptibility testing with VITEK 2 for the detection of vanA, vanB, and vanC1 strains was 100%. The sensitivity of vancomycin susceptibility testing of vanC2 strains was 77%. The sensitivity of teicoplanin susceptibility testing of vanA strains was 90%. Of 80 vanC enterococci, 78 (98%) were correctly identified by VITEK 2 as Enterococcus gallinarum/Enterococcus casseliflavus. Since the identification and susceptibility data are produced within 3 and 8 h, respectively, VITEK 2 appears a fast and reliable method for detection of GRE in microbiology laboratories.

* Corresponding author. Mailing address: Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: (31) 10 4635820. Fax: (31) 10 4633875. E-mail: vandenbraak{at}bacl.azr.nl.

Journal of Clinical Microbiology, January 2001, p. 351-353, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.351-353.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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