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Journal of Clinical Microbiology, January 2001, p. 362-364, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.362-364.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid and Specific Genotyping System for Hepatitis B Virus Corresponding to Six Major Genotypes by PCR Using Type-Specific Primers

Hideo Naito,1,2 Shigeki Hayashi,2 and Kenji Abe1,*

Department of Pathology, National Institute of Infectious Diseases,1 and Division of Gastroenterology, International Medical Center of Japan,2 Tokyo, Japan

Received 5 June 2000/Returned for modification 17 August 2000/Accepted 16 October 2000

A simple and precise genotyping system based on PCR using type-specific primers was developed for the determination of genotypes A through F of hepatitis B virus (HBV). This assay system is considered to be a useful tool for the molecular diagnosis of HBV infection and for large-scale surveys.

* Corresponding author. Mailing address: Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: (81) 3-5285-1111, ext. 2624. Fax: (81) 3-5285-1189. E-mail: kenjiabe{at}nih.go.jp.

Journal of Clinical Microbiology, January 2001, p. 362-364, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.362-364.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.