This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by La Scola, B.
Right arrow Articles by Raoult, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by La Scola, B.
Right arrow Articles by Raoult, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2001, p. 365-366, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.365-366.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation of Legionella anisa Using an Amoebic Coculture Procedure

Bernard La Scola,1 Lyazid Mezi,1 P. J. Weiller,2 and Didier Raoult1,*

Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine,1 and Service de Médecine Interne, Hôpital de la Timone,2 13385 Marseille Cedex 05, France

Received 7 August 2000/Returned for modification 2 October 2000/Accepted 31 October 2000

Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: 33.4.91.38.55.17. Fax: 33.4.91.83.03.90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, January 2001, p. 365-366, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.365-366.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Evstigneeva, A., Raoult, D., Karpachevskiy, L., La Scola, B. (2009). Amoeba co-culture of soil specimens recovered 33 different bacteria, including four new species and Streptococcus pneumoniae. Microbiology 155: 657-664 [Abstract] [Full Text]  
  • Adekambi, T., Ben Salah, S., Khlif, M., Raoult, D., Drancourt, M. (2006). Survival of Environmental Mycobacteria in Acanthamoeba polyphaga. Appl. Environ. Microbiol. 72: 5974-5981 [Abstract] [Full Text]  
  • Thomas, V., Herrera-Rimann, K., Blanc, D. S., Greub, G. (2006). Biodiversity of Amoebae and Amoeba-Resisting Bacteria in a Hospital Water Network. Appl. Environ. Microbiol. 72: 2428-2438 [Abstract] [Full Text]  
  • Adekambi, T., Reynaud-Gaubert, M., Greub, G., Gevaudan, M.-J., La Scola, B., Raoult, D., Drancourt, M. (2004). Amoebal Coculture of "Mycobacterium massiliense" sp. nov. from the Sputum of a Patient with Hemoptoic Pneumonia. J. Clin. Microbiol. 42: 5493-5501 [Abstract] [Full Text]  
  • Greub, G., Raoult, D. (2004). Microorganisms Resistant to Free-Living Amoebae. Clin. Microbiol. Rev. 17: 413-433 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»