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Journal of Clinical Microbiology, January 2001, p. 370-374, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.370-374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes

Tobias Bellin,1 Matthias Pulz,2 Andreas Matussek,1 Hans-Günther Hempen,1 and Florian Gunzer1,*

Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover,1 and Niedersächisches Landesgesundheitsamt, 30449 Hannover,2 Germany

Received 1 March 2000/Returned for modification 17 July 2000/Accepted 23 October 2000

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.


* Corresponding author. Mailing address: Hannover Medical School, Institute of Medical Microbiology, Carl-Neuberg Strasse 1, 30625 Hannover, Germany. Phone: 49 511 532 4352. Fax: 49 511 532 4366. E-mail: fgunzer{at}mikbio.h.shuttle.de.


Journal of Clinical Microbiology, January 2001, p. 370-374, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.370-374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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