This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bellin, T.
Right arrow Articles by Gunzer, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bellin, T.
Right arrow Articles by Gunzer, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2001, p. 370-374, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.370-374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes

Tobias Bellin,1 Matthias Pulz,2 Andreas Matussek,1 Hans-Günther Hempen,1 and Florian Gunzer1,*

Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover,1 and Niedersächisches Landesgesundheitsamt, 30449 Hannover,2 Germany

Received 1 March 2000/Returned for modification 17 July 2000/Accepted 23 October 2000

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

* Corresponding author. Mailing address: Hannover Medical School, Institute of Medical Microbiology, Carl-Neuberg Strasse 1, 30625 Hannover, Germany. Phone: 49 511 532 4352. Fax: 49 511 532 4366. E-mail: fgunzer{at}mikbio.h.shuttle.de.

Journal of Clinical Microbiology, January 2001, p. 370-374, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.370-374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhang, W., Bielaszewska, M., Pulz, M., Becker, K., Friedrich, A. W., Karch, H., Kuczius, T. (2008). New Immuno-PCR Assay for Detection of Low Concentrations of Shiga Toxin 2 and Its Variants. J. Clin. Microbiol. 46: 1292-1297 [Abstract] [Full Text]  
  • Yang, J.-R., Wu, F.-T., Tsai, J.-L., Mu, J.-J., Lin, L.-F., Chen, K.-L., Kuo, S. H.-S., Chiang, C.-S., Wu, H.-S. (2007). Comparison between O Serotyping Method and Multiplex Real-Time PCR To Identify Diarrheagenic Escherichia coli in Taiwan. J. Clin. Microbiol. 45: 3620-3625 [Abstract] [Full Text]  
  • Huang, L., Cooper, M. A. (2006). Real-time label-free acoustic technology for rapid detection of Escherichia coli O157:H7.. Clin. Chem. 52: 2148-2151 [Full Text]  
  • Nguyen, T. V., Le Van, P., Le Huy, C., Gia, K. N., Weintraub, A. (2005). Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam. J. Clin. Microbiol. 43: 755-760 [Abstract] [Full Text]  
  • Reischl, U., Youssef, M. T., Wolf, H., Hyytia-Trees, E., Strockbine, N. A. (2004). Real-Time Fluorescence PCR Assays for Detection and Characterization of Heat-Labile I and Heat-Stable I Enterotoxin Genes from Enterotoxigenic Escherichia coli. J. Clin. Microbiol. 42: 4092-4100 [Abstract] [Full Text]  
  • Kurupati, P., Chow, C., Kumarasinghe, G., Poh, C. L. (2004). Rapid Detection of Klebsiella pneumoniae from Blood Culture Bottles by Real-Time PCR. J. Clin. Microbiol. 42: 1337-1340 [Abstract] [Full Text]  
  • Fukushima, H., Tsunomori, Y., Seki, R. (2003). Duplex Real-Time SYBR Green PCR Assays for Detection of 17 Species of Food- or Waterborne Pathogens in Stools. J. Clin. Microbiol. 41: 5134-5146 [Abstract] [Full Text]  
  • Jinneman, K. C., Yoshitomi, K. J., Weagant, S. D. (2003). Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes stx1 and stx2 and Escherichia coli O157:H7/H- Serotype. Appl. Environ. Microbiol. 69: 6327-6333 [Abstract] [Full Text]  
  • Pulz, M., Matussek, A., Monazahian, M., Tittel, A., Nikolic, E., Hartmann, M., Bellin, T., Buer, J., Gunzer, F. (2003). Comparison of a Shiga Toxin Enzyme-Linked Immunosorbent Assay and Two Types of PCR for Detection of Shiga Toxin-Producing Escherichia coli in Human Stool Specimens. J. Clin. Microbiol. 41: 4671-4675 [Abstract] [Full Text]  
  • Miller, N., Cleary, T., Kraus, G., Young, A. K., Spruill, G., Hnatyszyn, H. J. (2002). Rapid and Specific Detection of Mycobacterium tuberculosis from Acid-Fast Bacillus Smear-Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real-Time PCR. J. Clin. Microbiol. 40: 4143-4147 [Abstract] [Full Text]  
  • Reischl, U., Youssef, M. T., Kilwinski, J., Lehn, N., Zhang, W. L., Karch, H., Strockbine, N. A. (2002). Real-Time Fluorescence PCR Assays for Detection and Characterization of Shiga Toxin, Intimin, and Enterohemolysin Genes from Shiga Toxin-Producing Escherichia coli. J. Clin. Microbiol. 40: 2555-2565 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»