JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bellin, T.
Right arrow Articles by Gunzer, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bellin, T.
Right arrow Articles by Gunzer, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2001, p. 370-374, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.370-374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes

Tobias Bellin,1 Matthias Pulz,2 Andreas Matussek,1 Hans-Günther Hempen,1 and Florian Gunzer1,*

Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover,1 and Niedersächisches Landesgesundheitsamt, 30449 Hannover,2 Germany

Received 1 March 2000/Returned for modification 17 July 2000/Accepted 23 October 2000

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.


* Corresponding author. Mailing address: Hannover Medical School, Institute of Medical Microbiology, Carl-Neuberg Strasse 1, 30625 Hannover, Germany. Phone: 49 511 532 4352. Fax: 49 511 532 4366. E-mail: fgunzer{at}mikbio.h.shuttle.de.


Journal of Clinical Microbiology, January 2001, p. 370-374, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.370-374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.