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Journal of Clinical Microbiology, January 2001, p. 381-384, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.381-384.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)

Didier Moissenet,1,* Philippe Bidet,1 Antoine Garbarg-Chenon,1 Guillaume Arlet,2 and Hoang Vu-Thien1

Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau,1 and Service de Microbiologie, Hôpital Tenon,2 Faculté de Médecine Saint Antoine, Université Paris VI, Paris, France

Received 11 July 2000/Returned for modification 29 August 2000/Accepted 24 October 2000

Ralstonia paucula (formerly CDC group IV c-2) can cause serious human infections. Confronted in 1995 with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, 26 Avenue du Dr Arnold-Netter, 75571 Paris Cedex 12, France. Phone: 33 1 44 73 61 43. Fax: 33 1 44 73 62 88. E-mail: didier.moissenet{at}trs.ap-hop-paris.fr.

Journal of Clinical Microbiology, January 2001, p. 381-384, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.381-384.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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