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Journal of Clinical Microbiology, January 2001, p. 398-400, Vol. 39, No. 1
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.1.398-400.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Rapid Identification of Virulent Type I Strains of
the Protozoan Pathogen Toxoplasma gondii by
PCR-Restriction Fragment Length Polymorphism Analysis at the
B1 Gene
Michael E.
Grigg and
John C.
Boothroyd*
Department of Microbiology and Immunology,
Stanford University School of Medicine, Stanford, California
94305-5124
Received 27 July 2000/Returned for modification 27 September
2000/Accepted 28 October 2000
Sequence analysis at the 35-fold-repetitive B1 locus
identified three restriction sites capable of discriminating type I
(mouse-virulent) from type II or III (mouse-avirulent) strains of
Toxoplasma gondii. B1 PCR-restriction fragment
length polymorphism analysis of 8 type I, 17 type II, and 8 type III
strains confirms the specificity of the assay. It should now be
possible to ask whether strain genotype affects the severity and type
of clinical disease in humans.
*
Corresponding author. Mailing address:Department of
Microbiology and Immunology, Stanford University School of Medicine,
Sherman Fairchild Building, D-305, 299 Campus Dr., Stanford, CA
94305-5124. Phone: (650) 723-7984. Fax: (650) 723-6853. E-mail:
jboothr{at}leland.stanford.edu.
Journal of Clinical Microbiology, January 2001, p. 398-400, Vol. 39, No. 1
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.1.398-400.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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