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Journal of Clinical Microbiology, January 2001, p. 398-400, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.398-400.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Identification of Virulent Type I Strains of the Protozoan Pathogen Toxoplasma gondii by PCR-Restriction Fragment Length Polymorphism Analysis at the B1 Gene

Michael E. Grigg and John C. Boothroyd*

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305-5124

Received 27 July 2000/Returned for modification 27 September 2000/Accepted 28 October 2000

Sequence analysis at the 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mouse-virulent) from type II or III (mouse-avirulent) strains of Toxoplasma gondii. B1 PCR-restriction fragment length polymorphism analysis of 8 type I, 17 type II, and 8 type III strains confirms the specificity of the assay. It should now be possible to ask whether strain genotype affects the severity and type of clinical disease in humans.

* Corresponding author. Mailing address:Department of Microbiology and Immunology, Stanford University School of Medicine, Sherman Fairchild Building, D-305, 299 Campus Dr., Stanford, CA 94305-5124. Phone: (650) 723-7984. Fax: (650) 723-6853. E-mail: jboothr{at}leland.stanford.edu.

Journal of Clinical Microbiology, January 2001, p. 398-400, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.398-400.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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