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Journal of Clinical Microbiology, January 2001, p. 90-93, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.90-93.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Coinfection with Three Ehrlichia Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure

Jiraporn Suksawat,1 Christian Pitulle,1 Cruz Arraga-Alvarado,2 Karina Madrigal,2 Susan I. Hancock,1 and Edward B. Breitschwerdt1,*

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606,1 and Unidad de Investigacions Clinicas, Facultad de Ciencias, Veterinarias, Universidad del Zulia, Maracaibo, Venezuela2

Received 2 August 2000/Returned for modification 8 September 2000/Accepted 25 October 2000

As part of a larger study to investigate tick-borne infections in dogs from Thailand and Venezuela, documentation of coinfection with three Ehrlichia species in two dogs, one from each country, became the focus of the present study. Although neither dog had clinical signs attributable to ehrlichiosis, both dogs were anemic and neutropenic and the Thai dog was thrombocytopenic. Genus- and species-specific PCR targeting the 16S rRNA genes indicated that both dogs were coinfected with Ehrlichia canis, E. platys, and E. equi. To our knowledge, these results provide the first molecular documentation for the presence of E. equi in dogs from these countries. Using universal bacterial PCR primers, one nearly full-length 16S rRNA gene could be amplified from each dog. The sequences were identical to each other and almost identical to that of E. platys (AF156784), providing the first E. platys 16S ribosomal DNA (rDNA) sequences reported from these two geographically divergent countries. To determine whether these sequence differences allow differentiation between these two strains and other published 16S rDNA E. platys sequences, we performed a phylogenetic analysis of the rRNA, incorporating the consideration of secondary structure.

* Corresponding author. Mailing address: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St., Raleigh, NC 27606. Phone: (919) 513-6234. Fax: (919) 513-6336. E-mail: ed_breitschwerdt{at}ncsu.edu.

Journal of Clinical Microbiology, January 2001, p. 90-93, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.90-93.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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