This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loparev, V. N.
Right arrow Articles by Meyer, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loparev, V. N.
Right arrow Articles by Meyer, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2001, p. 94-100, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.94-100.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection and Differentiation of Old World Orthopoxviruses: Restriction Fragment Length Polymorphism of the crmB Gene Region

Vladimir N. Loparev,1,* Robert F. Massung,1 Joseph J. Esposito,1 and Hermann Meyer2

Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia 30333,1 and Federal Armed Forces Medical Academy, Institute of Microbiology, D-80937 Munich, Germany2

Received 12 May 2000/Returned for modification 21 August 2000/Accepted 23 October 2000

A restriction fragment length polymorphism (RFLP) assay was developed to identify and differentiate Old World, African-Eurasian orthopoxviruses (OPV): variola, vaccinia, cowpox, monkeypox, camelpox, ectromelia, and taterapox viruses. The test uses amplicons produced from virus genome DNA by PCR with a consensus primer pair designed from sequences determined for the cytokine response modifier B (crmB) gene of 43 different OPV strains of known taxonomic origin. The primer pair amplified a single specific product from each of the 115 OPV samples tested. Size-specific amplicons identified and differentiated ectromelia and vaccinia virus strains, which contain a truncated crmB gene, and enabled their differentiation from other OPV species. Restriction digests of amplified products allowed the identification and differentiation of variola, monkeypox, camelpox, vaccinia, and cowpox virus species and strains.

* Corresponding author. Mailing address: Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, CDC, MS D-10, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-4040. Fax: (404) 639-4056. E-mail: vnl0{at}cdc.gov.

Journal of Clinical Microbiology, January 2001, p. 94-100, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.94-100.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Balamurugan, V., Jayappa, K. D., Hosamani, M., Bhanuprakash, V., Venkatesan, G., Singh, R. K. (2009). Comparative efficacy of conventional and taqman polymerase chain reaction assays in the detection of capripoxviruses from clinical samples. jvdi 21: 225-231 [Abstract] [Full Text]  
  • Scaramozzino, N., Ferrier-Rembert, A., Favier, A.-l., Rothlisberger, C., Richard, S., Crance, J.-M., Meyer, H., Garin, D. (2007). Real-Time PCR to Identify Variola Virus or Other Human Pathogenic Orthopox Viruses. Clin. Chem. 53: 606-613 [Abstract] [Full Text]  
  • Fedele, C. G., Negredo, A., Molero, F., Sanchez-Seco, M. P., Tenorio, A. (2006). Use of Internally Controlled Real-Time Genome Amplification for Detection of Variola Virus and Other Orthopoxviruses Infecting Humans. J. Clin. Microbiol. 44: 4464-4470 [Abstract] [Full Text]  
  • Olson, V. A., Laue, T., Laker, M. T., Babkin, I. V., Drosten, C., Shchelkunov, S. N., Niedrig, M., Damon, I. K., Meyer, H. (2004). Real-Time PCR System for Detection of Orthopoxviruses and Simultaneous Identification of Smallpox Virus. J. Clin. Microbiol. 42: 1940-1946 [Abstract] [Full Text]  
  • Nitsche, A., Ellerbrok, H., Pauli, G. (2004). Detection of Orthopoxvirus DNA by Real-Time PCR and Identification of Variola Virus DNA by Melting Analysis. J. Clin. Microbiol. 42: 1207-1213 [Abstract] [Full Text]  
  • Kulesh, D. A., Baker, R. O., Loveless, B. M., Norwood, D., Zwiers, S. H., Mucker, E., Hartmann, C., Herrera, R., Miller, D., Christensen, D., Wasieloski, L. P. Jr., Huggins, J., Jahrling, P. B. (2004). Smallpox and pan-Orthopox Virus Detection by Real-Time 3'-Minor Groove Binder TaqMan Assays on the Roche LightCycler and the Cepheid Smart Cycler Platforms. J. Clin. Microbiol. 42: 601-609 [Abstract] [Full Text]  
  • Sofi Ibrahim, M., Kulesh, D. A., Saleh, S. S., Damon, I. K., Esposito, J. J., Schmaljohn, A. L., Jahrling, P. B. (2003). Real-Time PCR Assay To Detect Smallpox Virus. J. Clin. Microbiol. 41: 3835-3839 [Abstract] [Full Text]  
  • Lapa, S., Mikheev, M., Shchelkunov, S., Mikhailovich, V., Sobolev, A., Blinov, V., Babkin, I., Guskov, A., Sokunova, E., Zasedatelev, A., Sandakhchiev, L., Mirzabekov, A. (2002). Species-Level Identification of Orthopoxviruses with an Oligonucleotide Microchip. J. Clin. Microbiol. 40: 753-757 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»