This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mulvey, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mulvey, M. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2001, p. 3481-3485, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3481-3485.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of a Canadian Standardized Protocol for Subtyping Methicillin-Resistant Staphylococcus aureus Using Pulsed-Field Gel Electrophoresis

M. R. Mulvey,1,* L. Chui,2 J. Ismail,3 L. Louie,4 C. Murphy,1 N. Chang,2 M. Alfa,5 and the Canadian Committee for the Standardization of Molecular Methodsdagger

National Microbiology Laboratory, Health Canada,1 and St. Boniface Hospital,5 Winnipeg, Manitoba, Alberta Provincial Laboratory, Edmonton, Alberta,2 LSPQ/INSPQ, SainteAnne de Bellevue, Quebec,3 and Sunnybrook and Women's College and Health Science Centre, Toronto, Ontario,4 Canada

Received 1 May 2001/Returned for modification 30 May 2001/Accepted 22 July 2001

A panel of 24 methicillin-resistant Staphylococcus aureus strains was distributed to 15 laboratories in Canada to evaluate their in-house pulsed-field gel electrophoresis (PFGE) protocols and interpretation criteria. Attempts to compare fingerprint images using computer-aided analysis were not successful due to variability in individual laboratory PFGE protocols. In addition, individual site interpretation of the fingerprint patterns was inadequate, as 7 of 13 sites (54%) made at least one error in interpreting the fingerprints from the panel. A 2-day standardized PFGE protocol (culture to gel image) was developed and distributed to all of the sites. Each site was requested to use the standardized protocol on five strains from the original panel. Thirteen sites submitted gel images for comparisons. The protocol demonstrated excellent reproducibility and allowed interlaboratory comparisons with Molecular Analyst DST software (Bio-Rad) and 1.5% band tolerance.

* Corresponding author. Mailing address: National Microbiology Laboratory, Nosocomial Infections, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2133. Fax: (204) 789-2018. E-mail: michael_mulvey{at}he-sc.gc.ca.

dagger Participants of The Canadian Committee for the Standardization of Molecular Methods: L. Abbott, Charlottetown, Prince Edward Island; M. Alfa, Winnipeg, Manitoba; S. Byrne, Vancouver, British Columbia; B. Ciebin, Toronto, Ontario; L. Chui, Edmonton, Alberta; C. Clark, Winnipeg, Manitoba; J. Conly, Toronto, Ontario; R. Davidson, Halifax, Nova Scotia; J. Farber, Ottowa, Ontario; K. Fonseca, Calgary, Alberta; G. Horsman, Regina, Saskatchwan; J. Ismail, Ste-Anne de Bellevue, Quebec; P. Jayaratne, Hamilton, Ontario; A. Kabani, Winnipeg, Manitoba; M. Kanchana, Saskatoon, Saskatchewan; L. Louie, Toronto, Ontario; A. McGeer, Toronto, Ontario; M. Mulvey, Winnipeg, Manitoba; G. Norris, St. John's, Newfoundland; M. Peppler, Edmonton, Alberta; G. Peters, London, Ontario; K. Ramotar, Ottowa, Ontario; J. Wylie, Winnipeg, Manitoba; and K. Ziebell, Guelph, Ontario.

Journal of Clinical Microbiology, October 2001, p. 3481-3485, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3481-3485.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Adam, H. J., Laing, N. M., King, C. R., Lulashnyk, B., Hoban, D. J., Zhanel, G. G. (2009). In Vitro Activity of Nemonoxacin, a Novel Nonfluorinated Quinolone, against 2,440 Clinical Isolates. Antimicrob. Agents Chemother. 53: 4915-4920 [Abstract] [Full Text]  
  • Walther, B., Monecke, S., Ruscher, C., Friedrich, A. W., Ehricht, R., Slickers, P., Soba, A., Wleklinski, C.-G., Wieler, L. H., Lubke-Becker, A. (2009). Comparative Molecular Analysis Substantiates Zoonotic Potential of Equine Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 47: 704-710 [Abstract] [Full Text]  
  • Antoniou, T, Devlin, R, Gough, K, Mulvey, M, Katz, K C, Zehtabchi, M, Polsky, J, Tilley, D, Brunetta, J, Arbess, G, Guiang, C, Chang, B, Kovacs, C, Ghavam-Rassoul, A, Cavacuiti, C, Corneslon, B, Berger, P, Loutfy, M R (2009). Prevalence of community-associated methicillin-resistant Staphylococcus aureus colonization in men who have sex with men. Int J STD AIDS 20: 180-183 [Abstract] [Full Text]  
  • Zhang, K., McClure, J.-A., Elsayed, S., Conly, J. M. (2009). Novel Staphylococcal Cassette Chromosome mec Type, Tentatively Designated Type VIII, Harboring Class A mec and Type 4 ccr Gene Complexes in a Canadian Epidemic Strain of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 53: 531-540 [Abstract] [Full Text]  
  • Zhanel, G. G., DeCorby, M., Laing, N., Weshnoweski, B., Vashisht, R., Tailor, F., Nichol, K. A., Wierzbowski, A., Baudry, P. J., Karlowsky, J. A., Lagace-Wiens, P., Walkty, A., McCracken, M., Mulvey, M. R., Johnson, J., The Canadian Antimicrobial Resistance Alliance (CA, , Hoban, D. J. (2008). Antimicrobial-Resistant Pathogens in Intensive Care Units in Canada: Results of the Canadian National Intensive Care Unit (CAN-ICU) Study, 2005-2006. Antimicrob. Agents Chemother. 52: 1430-1437 [Abstract] [Full Text]  
  • Zhang, K., McClure, J.-A., Elsayed, S., Louie, T., Conly, J. M. (2008). Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci. J. Clin. Microbiol. 46: 1118-1122 [Abstract] [Full Text]  
  • Christianson, S., Golding, G. R., Campbell, J., the Canadian Nosocomial Infection Surveillance Pro, , Mulvey, M. R. (2007). Comparative Genomics of Canadian Epidemic Lineages of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 1904-1911 [Abstract] [Full Text]  
  • Karlowsky, J. A., Laing, N. M., Baudry, T., Kaplan, N., Vaughan, D., Hoban, D. J., Zhanel, G. G. (2007). In Vitro Activity of API-1252, a Novel FabI Inhibitor, against Clinical Isolates of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob. Agents Chemother. 51: 1580-1581 [Full Text]  
  • Revazishvili, T., Bakanidze, L., Gomelauri, T., Zhgenti, E., Chanturia, G., Kekelidze, M., Rajanna, C., Kreger, A., Sulakvelidze, A. (2006). Genetic Background and Antibiotic Resistance of Staphylococcus aureus Strains Isolated in the Republic of Georgia.. J. Clin. Microbiol. 44: 3477-3483 [Abstract] [Full Text]  
  • Gilbert, M., MacDonald, J., Gregson, D., Siushansian, J., Zhang, K., Elsayed, S., Laupland, K., Louie, T., Hope, K., Mulvey, M., Gillespie, J., Nielsen, D., Wheeler, V., Louie, M., Honish, A., Keays, G., Conly, J. (2006). Outbreak in Alberta of community-acquired (USA300) methicillin-resistant Staphylococcus aureus in people with a history of drug use, homelessness or incarceration. CMAJ 175: 149-154 [Abstract] [Full Text]  
  • Aires-de-Sousa, M., Boye, K., de Lencastre, H., Deplano, A., Enright, M. C., Etienne, J., Friedrich, A., Harmsen, D., Holmes, A., Huijsdens, X. W., Kearns, A. M., Mellmann, A., Meugnier, H., Rasheed, J. K., Spalburg, E., Strommenger, B., Struelens, M. J., Tenover, F. C., Thomas, J., Vogel, U., Westh, H., Xu, J., Witte, W. (2006). High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study. J. Clin. Microbiol. 44: 619-621 [Abstract] [Full Text]  
  • Hardy, K. J., Oppenheim, B. A., Gossain, S., Gao, F., Hawkey, P. M. (2006). Use of Variations in Staphylococcal Interspersed Repeat Units for Molecular Typing of Methicillin-Resistant Staphylococcus aureus Strains. J. Clin. Microbiol. 44: 271-273 [Abstract] [Full Text]  
  • Lu, J.-J., Lee, S.-Y., Hwa, S.-Y., Yang, A.-H. (2005). Septic Arthritis Caused by Vancomycin-Intermediate Staphylococcus aureus. J. Clin. Microbiol. 43: 4156-4158 [Abstract] [Full Text]  
  • Malachowa, N., Sabat, A., Gniadkowski, M., Krzyszton-Russjan, J., Empel, J., Miedzobrodzki, J., Kosowska-Shick, K., Appelbaum, P. C., Hryniewicz, W. (2005). Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Pulsed-Field Gel Electrophoresis, spa Typing, and Multilocus Sequence Typing for Clonal Characterization of Staphylococcus aureus Isolates. J. Clin. Microbiol. 43: 3095-3100 [Abstract] [Full Text]  
  • Wylie, J. L., Nowicki, D. L. (2005). Molecular Epidemiology of Community- and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Manitoba, Canada. J. Clin. Microbiol. 43: 2830-2836 [Abstract] [Full Text]  
  • Northey, G., Gal, M., Rahmati, A., Brazier, J. S (2005). Subtyping of Clostridium difficile PCR ribotype 001 by REP-PCR and PFGE. J Med Microbiol 54: 543-547 [Abstract] [Full Text]  
  • Weese, J. S. (2005). Methicillin-Resistant Staphylococcus aureus: An Emerging Pathogen in Small Animals. Journal of the American Animal Hospital Association 41: 150-157 [Abstract] [Full Text]  
  • Murchan, S., Kaufmann, M. E., Deplano, A., de Ryck, R., Struelens, M., Zinn, C. E., Fussing, V., Salmenlinna, S., Vuopio-Varkila, J., El Solh, N., Cuny, C., Witte, W., Tassios, P. T., Legakis, N., van Leeuwen, W., van Belkum, A., Vindel, A., Laconcha, I., Garaizar, J., Haeggman, S., Olsson-Liljequist, B., Ransjo, U., Coombes, G., Cookson, B. (2003). Harmonization of Pulsed-Field Gel Electrophoresis Protocols for Epidemiological Typing of Strains of Methicillin-Resistant Staphylococcus aureus: a Single Approach Developed by Consensus in 10 European Laboratories and Its Application for Tracing the Spread of Related Strains. J. Clin. Microbiol. 41: 1574-1585 [Abstract] [Full Text]  
  • Peacock, S. J., de Silva, G. D. I., Justice, A., Cowland, A., Moore, C. E., Winearls, C. G., Day, N. P. J. (2002). Comparison of Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis as Tools for Typing Staphylococcus aureus Isolates in a Microepidemiological Setting. J. Clin. Microbiol. 40: 3764-3770 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»