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Journal of Clinical Microbiology, October 2001, p. 3603-3608, Vol. 39, No. 10
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3603-3608.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Clinical Evaluation of Anti-Tuberculous Glycolipid
Immunoglobulin G Antibody Assay for Rapid Serodiagnosis of
Pulmonary Tuberculosis
Ryoji
Maekura,1,*
Yoshinari
Okuda,1
Masaru
Nakagawa,1
Touru
Hiraga,1
Souichirou
Yokota,1
Masami
Ito,1
Ikuya
Yano,2
Hiroaki
Kohno,3
Masako
Wada,4
Chiyoji
Abe,4
Takeo
Toyoda,5
Toshio
Kishimoto,6 and
Takeshi
Ogura1
Toneyama National
Hospital,1 and Department of
Bacteriology, Osaka City University Medical
School,2 Osaka, Fuji Research
Laboratories, Kyowa Medex Co., Ltd., Shizuoka,3
Research Institution of Tuberculosis, Japan
Anti-Tuberculosis Association, Tokyo,4
Higashi-Saitama National Hospital,
Saitama,5 and Department of
Health Care Medicine, Kawasaki Medical School,
Okayama,6 Japan
Received 30 April 2001/Returned for modification 9 June
2001/Accepted 15 July 2001
Previously we reported the development of a highly sensitive
enzyme-linked immunosorbent assay specific for anti-tuberculous glycolipid (anti-TBGL) for the rapid serodiagnosis of tuberculosis. In
this study, the usefulness of an anti-TBGL antibody assay kit for rapid
serodiagnosis was evaluated in a controlled multicenter study. Antibody
titers in sera from 318 patients with active pulmonary tuberculosis (216 positive for Mycobacterium
tuberculosis in smear and/or culture tests and 102 smear and
culture negative and clinically diagnosed), 58 patients with old
tuberculosis, 177 patients with other respiratory diseases, 156 patients with nonrespiratory diseases, and 454 healthy subjects were
examined. Sera from 256 younger healthy subjects from among the 454 healthy subjects were examined as a control. When the cutoff point of
anti-TBGL antibody titer was determined as 2.0 U/ml, the sensitivity
for active tuberculosis patients was 81.1% and the specificity was
95.7%. Sensitivity in patients with smear-negative and
culture-negative active pulmonary tuberculosis was 73.5%. Even in
patients with noncavitary minimally advanced lesions, the positivity
rate (60.0%) and the antibody titer (4.6 ± 9.4 U/ml) were
significantly higher than those in the healthy group. These results
indicate that this assay using anti-TBGL antibody is useful for
the rapid serodiagnosis of active pulmonary tuberculosis.
*
Corresponding author. Mailing address: Toneyama
National Hospital, 5-1-1 Toneyama, Toyonaka-City, Osaka 560-0045, Japan. Phone: 81-6-6853-2001. Fax: 81-6-6853-3127. E-mail:
maekurar{at}med.osaka-cu.ac.jp.
Journal of Clinical Microbiology, October 2001, p. 3603-3608, Vol. 39, No. 10
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3603-3608.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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