JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsheka, M. I.
Right arrow Articles by Elisha, B. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsheka, M. I.
Right arrow Articles by Elisha, B. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2001, p. 3684-3689, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3684-3689.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Identification of Campylobacter concisus

M. I. Matsheka,1,dagger A. J. Lastovica,1,2 and B. Gay Elisha1,2,*

Department of Medical Microbiology, University of Cape Town,1 and Goote Schuur Hospital,2 Cape Town, South Africa

Received 6 March 2001/Returned for modification 2 May 2001/Accepted 15 July 2001

A 1.6-kb DNA fragment isolated from a Campylobacter concisus genomic library gave C. concisus-specific restriction fragment length patterns when it was used as a probe in hybridization studies. All of the strains tested, including type strains and clinical isolates, contained a 0.5-kb HindIII fragment that hybridized to the probe. DNA sequencing of the 1.6-kb fragment identified three open reading frames (ORFs). One of the ORFs encodes the carboxy terminus of GyrB, and the translational products of ORF2 and ORF3 showed similarity to hypothetical proteins, previously identified in Campylobacter jejuni. DNA-DNA hybridization studies with a fragment internal to ORF3 showed that this sequence was responsible for the signal observed with the 0.5-kb HindIII fragment. A rapid PCR assay was developed and evaluated. Primers that annealed to the extremities of the 1.6-kb fragment were used to obtain an amplicon of the correct size from both reference and clinical strains of C. concisus.

* Corresponding author. Mailing address: Department of Medical Microbiology, Medical School, UCT, Anzio Rd., Observatory 7925, Cape Town, South Africa. Phone: (021) 4066378. Fax: (021) 4488153. E-mail: gelisha{at}curie.uct.ac.za.

dagger Present address: Department of Biological Sciences, University of Botswana, Gaborone, Botswana.

Journal of Clinical Microbiology, October 2001, p. 3684-3689, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3684-3689.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.