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Journal of Clinical Microbiology, October 2001, p. 3690-3692, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3690-3692.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Serotype-Specific Detection of Coxsackievirus A16 in Clinical Specimens by Reverse Transcription-Nested PCR

Justin W. A. Bendig,1,* Patrick S. O'Brien,1 and Peter Muir2

PHLS Coxsackievirus Reference Unit, Epsom, Surrey,1 and Department of Infection, Guy's, King's, and St. Thomas' School of Medicine, King's College London, London,2 United Kingdom

Received 7 March 2001/Returned for modification 11 June 2001/Accepted 10 July 2001

We describe the development of a coxsackievirus A16 (CVA16) serotype-specific PCR which correctly differentiated between CVA16 and other enterovirus serotypes of both laboratory isolates and clinical specimens. The assay will be useful for monitoring CVA16 outbreaks and studying the disease association, epidemiology, and evolution of this common enterovirus serotype.

* Corresponding author. Mailing address: PHLS Coxsackievirus Reference Unit, Department of Medical Microbiology, West Park Hospital, Horton La., Epsom, Surrey KT19 8PB, United Kingdom. Phone: 44 (0)1372-734700. Fax: 44 (0)1372-743619. E-mail: justin.bendig{at}virgin.net.

Journal of Clinical Microbiology, October 2001, p. 3690-3692, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3690-3692.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, L., He, Y., Yang, H., Zhu, J., Xu, X., Dong, J., Zhu, Y., Jin, Q. (2005). Genetic Characteristics of Human Enterovirus 71 and Coxsackievirus A16 Circulating from 1999 to 2004 in Shenzhen, People's Republic of China. J. Clin. Microbiol. 43: 3835-3839 [Abstract] [Full Text]  
  • Singh, S., Chow, V. T. K., Phoon, M. C., Chan, K. P., Poh, C. L. (2002). Direct Detection of Enterovirus 71 (EV71) in Clinical Specimens from a Hand, Foot, and Mouth Disease Outbreak in Singapore by Reverse Transcription-PCR with Universal Enterovirus and EV71-Specific Primers. J. Clin. Microbiol. 40: 2823-2827 [Abstract] [Full Text]  


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