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Journal of Clinical Microbiology, October 2001, p. 3693-3695, Vol. 39, No. 10
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3693-3695.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification of Staphylococcus spp. by
PCR-Restriction Fragment Length Polymorphism of gap
Gene
Javier
Yugueros,1
Alejandro
Temprano,1
María
Sánchez,1
José María
Luengo,2 and
Germán
Naharro1,*
Departamento de Sanidad Animal,
Microbiología e Inmunología1 and
Departamento de Bioquímica y Biología
Molecular,2 Universidad de León, 24071 León, Spain
Received 2 February 2001/Returned for modification 17 June
2001/Accepted 23 July 2001
Oligonucleotide primers specific for the Staphylococcus
aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study,
AluI digestion of PCR-generated products rendered
distinctive restriction fragment length polymorphism patterns that
allowed 24 Staphylococcus spp. to be identified with high specificity.
*
Corresponding author. Mailing address:
Departamento de Sanidad Animal, Microbiología e
Immunología Universidad de León, 24071 León, Spain.
Phone: 34-87-291294. Fax: 34-87-291304. E-mail: dsagnc{at}unileon.es.
Journal of Clinical Microbiology, October 2001, p. 3693-3695, Vol. 39, No. 10
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3693-3695.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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