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Journal of Clinical Microbiology, October 2001, p. 3693-3695, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3693-3695.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism of gap Gene

Javier Yugueros,1 Alejandro Temprano,1 María Sánchez,1 José María Luengo,2 and Germán Naharro1,*

Departamento de Sanidad Animal, Microbiología e Inmunología1 and Departamento de Bioquímica y Biología Molecular,2 Universidad de León, 24071 León, Spain

Received 2 February 2001/Returned for modification 17 June 2001/Accepted 23 July 2001

Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.

* Corresponding author. Mailing address: Departamento de Sanidad Animal, Microbiología e Immunología Universidad de León, 24071 León, Spain. Phone: 34-87-291294. Fax: 34-87-291304. E-mail: dsagnc{at}unileon.es.

Journal of Clinical Microbiology, October 2001, p. 3693-3695, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3693-3695.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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