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Journal of Clinical Microbiology, October 2001, p. 3700-3702, Vol. 39, No. 10
Institut für Medizinische Mikrobiologie
und Immunologie, Universitätsklinikum Hamburg-Eppendorf, D-20246
Hamburg, Germany
Received 29 May 2001/Returned for modification 4 July 2001/Accepted 15 July 2001
The detection of PBP 2a by the MRSA-Screen latex agglutination test
with 201 clinical coagulase-negative staphylococci had an
initial sensitivity of 98% and a high degree of specificity for
Staphylococcus epidermidis strains compared to PCR for
mecA. Determination of oxacillin MICs evaluated
according to the new breakpoint (0.5 µg/ml) of the National Committee
for Clinical Laboratory Standards exhibited an extremely low
specificity for this population.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3700-3702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Rapid Detection of Methicillin Resistance in Coagulase-Negative
Staphylococci by a Penicillin-Binding Protein 2a-Specific Latex
Agglutination Test
*
Corresponding author. Mailing address: Institut
für Medizinische Mikrobiologie und Immunologie,
Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Phone: 49 40 42803 2143. Fax: 49 40 42803 4881. E-mail: dmack{at}uke.uni-hamburg.de.
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