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Journal of Clinical Microbiology, October 2001, p. 3700-3702, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3700-3702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci by a Penicillin-Binding Protein 2a-Specific Latex Agglutination Test

Matthias A. Horstkotte, Johannes K.-M. Knobloch, Holger Rohde, and Dietrich Mack*

Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany

Received 29 May 2001/Returned for modification 4 July 2001/Accepted 15 July 2001

The detection of PBP 2a by the MRSA-Screen latex agglutination test with 201 clinical coagulase-negative staphylococci had an initial sensitivity of 98% and a high degree of specificity for Staphylococcus epidermidis strains compared to PCR for mecA. Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 µg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population.


* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Phone: 49 40 42803 2143. Fax: 49 40 42803 4881. E-mail: dmack{at}uke.uni-hamburg.de.


Journal of Clinical Microbiology, October 2001, p. 3700-3702, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3700-3702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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