This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Horstkotte, M. A.
Right arrow Articles by Mack, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horstkotte, M. A.
Right arrow Articles by Mack, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2001, p. 3700-3702, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3700-3702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci by a Penicillin-Binding Protein 2a-Specific Latex Agglutination Test

Matthias A. Horstkotte, Johannes K.-M. Knobloch, Holger Rohde, and Dietrich Mack*

Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany

Received 29 May 2001/Returned for modification 4 July 2001/Accepted 15 July 2001

The detection of PBP 2a by the MRSA-Screen latex agglutination test with 201 clinical coagulase-negative staphylococci had an initial sensitivity of 98% and a high degree of specificity for Staphylococcus epidermidis strains compared to PCR for mecA. Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 µg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Phone: 49 40 42803 2143. Fax: 49 40 42803 4881. E-mail: dmack{at}uke.uni-hamburg.de.

Journal of Clinical Microbiology, October 2001, p. 3700-3702, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3700-3702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Join-Lambert, O. F., Clauser, S., Guillet, C., Jais, J.-P., Abachin, E., Quesnes, G., Carbonnelle, E., Le Monnier, A., Zahar, J.-R., Kayal, S., Berche, P., Ferroni, A. (2007). Comparison of cefoxitin and moxalactam 30 {micro}g disc diffusion methods for detection of methicillin resistance in coagulase-negative staphylococci. J Antimicrob Chemother 59: 763-766 [Abstract] [Full Text]  
  • Perazzi, B., Fermepin, M. R., Malimovka, A., Garcia, S. D., Orgambide, M., Vay, C. A., de Torres, R., Famiglietti, A. M. R. (2006). Accuracy of cefoxitin disk testing for characterization of oxacillin resistance mediated by penicillin-binding protein 2a in coagulase-negative staphylococci.. J. Clin. Microbiol. 44: 3634-3639 [Abstract] [Full Text]  
  • Miller, M. B., Meyer, H., Rogers, E., Gilligan, P. H. (2005). Comparison of Conventional Susceptibility Testing, Penicillin-Binding Protein 2a Latex Agglutination Testing, and mecA Real-Time PCR for Detection of Oxacillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococcus. J. Clin. Microbiol. 43: 3450-3452 [Abstract] [Full Text]  
  • Horstkotte, M. A., Knobloch, J. K.-M., Rohde, H., Dobinsky, S., Mack, D. (2004). Evaluation of the BD PHOENIX Automated Microbiology System for Detection of Methicillin Resistance in Coagulase-Negative Staphylococci. J. Clin. Microbiol. 42: 5041-5046 [Abstract] [Full Text]  
  • Kipp, F., Becker, K., Peters, G., von Eiff, C. (2004). Evaluation of Different Methods To Detect Methicillin Resistance in Small-Colony Variants of Staphylococcus aureus. J. Clin. Microbiol. 42: 1277-1279 [Abstract] [Full Text]  
  • Horstkotte, M. A., Knobloch, J. K.-M., Rohde, H., Dobinsky, S., Mack, D. (2002). Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System. J. Clin. Microbiol. 40: 3291-3295 [Abstract] [Full Text]  
  • Hussain, Z., Stoakes, L., John, M. A., Garrow, S., Fitzgerald, V. (2002). Detection of Methicillin Resistance in Primary Blood Culture Isolates of Coagulase-Negative Staphylococci by PCR, Slide Agglutination, Disk Diffusion, and a Commercial Method. J. Clin. Microbiol. 40: 2251-2253 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»