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Journal of Clinical Microbiology, October 2001, p. 3705-3708, Vol. 39, No. 10
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3705-3708.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Rapid Differentiation of "Mycobacterium
canettii" from Other Mycobacterium
tuberculosis Complex Organisms by PCR-Restriction Analysis
of the hsp65 Gene
Khye Seng
Goh,
Eric
Legrand,
Christophe
Sola, and
Nalin
Rastogi*
Unité de la Tuberculose et des
Mycobactéries, Institut Pasteur de Guadeloupe, BP 484, F-97165 Pointe-à-Pitre Cedex, Guadeloupe
Received 15 February 2001/Returned for modification 13 May
2001/Accepted 1 July 2001
A total of 102 isolates of the Mycobacterium
tuberculosis complex, including available "M.
canettii" isolates, were studied by PCR-restriction analysis
of a 441-bp fragment of the hsp65 gene. PRA upon
HhaI enzyme digestion (GCGC) allowed easy
differentiatiation of "M. canettii" from other
members of the M. tuberculosis complex (three bands of
260, 105, and 60 bp for "M. canetti," compared to
four bands of 185, 105, 75, and 60 bp for other members of the
M. tuberculosis complex). Sequencing of the 441-bp
hsp65 fragment of "M. canettii"
isolates showed the disappearance of an HhaI site at
position 235 due to a C-to-T transition that corresponded to position
631 of the homologous hsp65 gene of M.
tuberculosis H37Rv. Considering that "M.
canettii" may also exist as a stable rough morphotype, we
suggest that the true number of "M. canettii" isolates may be underestimated in clinical microbiology laboratories.
*
Corresponding author. Mailing address: Institut
Pasteur, Morne Jolivière, BP 484, F-97165 Pointe-à-Pitre,
Cedex, Guadeloupe. Phone: 590-89 76 61. Fax: 590-89 38 80. E-mail:
rastogi{at}pasteur.gp.
Journal of Clinical Microbiology, October 2001, p. 3705-3708, Vol. 39, No. 10
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3705-3708.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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