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Journal of Clinical Microbiology, October 2001, p. 3709-3711, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3709-3711.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Epidemiologic Usefulness of Spoligotyping for Secondary Typing of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110

Wendy A. Cronin,1,* Jonathan E. Golub,1 Laurence S. Magder,2 Nancy G. Baruch,1 Monica J. Lathan,1 Leonard N. Mukasa,1 Nancy Hooper,3 Jafar H. Razeq,3 Donna Mulcahy,4 William H. Benjamin,5 and William R. Bishai6

Division of TB Control, Refugee and Migrant Health,1 and Public Health Microbiology, Laboratories Administration,3 Maryland Department of Health and Mental Hygiene, and Department of Epidemiology and Preventive Medicine, University of Maryland---Baltimore,2 Baltimore, Maryland 21201; Department of International Health, School of Public Health and Hygiene, Johns Hopkins University, Baltimore, Maryland 212056; Respiratory Disease Division, Department of Public Health, Bureau of Clinical Laboratories, Montgomery, Alabama 36130-30174; and Department of Pathology, University of Alabama, Birmingham, Alabama 352945

Received 9 April 2001/Returned for modification 15 June 2001/Accepted 6 August 2001

Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (<= 5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.

* Corresponding author. Mailing address: Division of TB Control, Refugee and Migrant Health, Maryland Department of Health and Mental Hygiene, 201 West Preston St., Rm. 307A, Baltimore, MD 21201. Phone: (410) 767-6693. Fax: (410) 669-4215. E-mail: croninw{at}dhmh.state.md.us.

Journal of Clinical Microbiology, October 2001, p. 3709-3711, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3709-3711.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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