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Journal of Clinical Microbiology, October 2001, p. 3712-3717, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3712-3717.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

PCR for Specific Detection of H7 Flagellar Variant of fliC among Extraintestinal Pathogenic Escherichia coli

James R. Johnson* and Adam L. Stelldagger

Medical Service, VA Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota

Received 26 March 2001/Returned for modification 20 June 2001/Accepted 18 July 2001

A newly developed PCR-based assay for the H7 variant of the Escherichia coli flagellin gene, fliC, was 100% sensitive and specific in comparison with serology and probe hybridization. It revealed broad conservation of the H7 fliC variant among phylogenetically diverse lineages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7 serotyping results. The H7 primers functioned well when incorporated into a multiplex PCR assay for diverse virulence-associated genes of ExPEC.

* Corresponding author. Mailing address: Infectious Diseases (111F), Minneapolis VA Medical Center, One Veterans Dr., Minneapolis, MN 55417. Phone: (612) 725-2000, ext. 4185. Fax: (612) 727-5995. E-mail: johns007{at}tc.umn.edu.

dagger Present address: Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin.

Journal of Clinical Microbiology, October 2001, p. 3712-3717, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3712-3717.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.