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Journal of Clinical Microbiology, October 2001, p. 3712-3717, Vol. 39, No. 10
Medical Service, VA Medical Center, and
Department of Medicine, University of Minnesota, Minneapolis, Minnesota
Received 26 March 2001/Returned for modification 20 June
2001/Accepted 18 July 2001
A newly developed PCR-based assay for the H7 variant of the
Escherichia coli flagellin gene,
fliC, was 100% sensitive and specific in
comparison with serology and probe hybridization. It revealed broad
conservation of the H7 fliC variant among
phylogenetically diverse lineages of extraintestinal pathogenic
E. coli (ExPEC) and superseded serotyping for certain
isolates with ambiguous or non-H7 serotyping results. The H7 primers
functioned well when incorporated into a multiplex PCR assay for
diverse virulence-associated genes of ExPEC.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3712-3717.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
PCR for Specific Detection of H7 Flagellar Variant
of fliC among Extraintestinal Pathogenic
Escherichia coli
*
Corresponding author. Mailing address: Infectious
Diseases (111F), Minneapolis VA Medical Center, One Veterans Dr.,
Minneapolis, MN 55417. Phone: (612) 725-2000, ext. 4185. Fax: (612)
727-5995. E-mail: johns007{at}tc.umn.edu.
Present address: Department of Molecular Genetics and Microbiology,
University of Texas at Austin, Austin.
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