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Journal of Clinical Microbiology, October 2001, p. 3721-3723, Vol. 39, No. 10
Department of Pediatrics, State University of
New York Downstate Medical Center, Brooklyn, New
York,1 and Center for Molecular
Biotechnology, Queensland University of Technology, Brisbane,
Queensland, Australia2
Received 9 February 2001/Returned for modification 20 June
2001/Accepted 25 July 2001
We have established an in vitro model of long-term continuous
Chlamydia pneumoniae infection in HEp-2 cells. Using
transmission electron microscopy, we demonstrated the presence of
spontaneous abnormal chlamydial inclusions similar in appearance to the
persistent chlamydial forms induced in vitro by treatment with
cytokines or antibiotics or by nutrient deprivation.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3721-3723.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Ultrastructural Study of Chlamydia
pneumoniae In a Continuous-Infection Model
*
Corresponding author. Mailing address: Department of
Pediatrics, Box 49, SUNY Downstate Medical Center at Brooklyn, 450 Clarkson Ave., Brooklyn, NY 11203-2098. Phone: (718) 245-4075. Fax:
(718) 245-2118. E-mail: mhammmerschlag{at}pol.net.
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