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Journal of Clinical Microbiology, October 2001, p. 3736-3739, Vol. 39, No. 10
Department of Respiratory Medicine, School of
Medicine, Semmelweis University,1 and
Korányi National Institute for Tuberculosis and
Respiratory Medicine,4 Budapest, Prodia
Laboratory for Mycobacteria, Jósa Hospital,
Nyíregyháza,3
Borsod-Abaúj-Zeplén County Bureau of Public Health
and Medical Officers, Miskolc,5 and
Laboratory for Mycobacteria, School of Medicine, University of
Debrecen, Debrecen,6 Hungary, and
Wadsworth Center, New York State Department of
Health,2 Department of Medicine, Albany
Medical College,7 and Department of
Biomedical Sciences, School of Public Health, State University of
New York at Albany,8 Albany, New York
Received 24 January 2001/Returned for modification 2 May
2001/Accepted 30 May 2001
Two regions of rpoB associated with rifampin
resistance were sequenced in 29 rifampin-resistant (determined by the
proportion method) isolates of Mycobacterium
tuberculosis obtained from patients from three counties in
Hungary. Of the 29 resistant strains, 27 had a mutation in either the
81-bp region (26 strains) or the N-terminal region (1 strain), while
the other 2 strains had no mutations in either region. The locations
and frequencies of the mutations differed from those previously
reported. The most common mutation in this study, D516V, was found in
38% of the Hungarian strains, a frequency 2 to 10 times higher than
that found in studies from other countries. These same 29 isolates were
also evaluated with the Inno-LiPA Rif. TB test (LiPA), a reverse
hybridization assay for the rapid detection of rifampin resistance.
Although LiPA detected the presence of an rpoB mutation
in 26 of the resistant isolates, the type of mutation could not be
determined in 4 isolates because the mutations present were not among
those included on the LiPA strip. In addition, a silent mutation in one
of the rifampin-susceptible control strains was interpreted as rifampin
resistant by LiPA. These findings demonstrate the importance of
validating this rapid molecular test by comparison with DNA sequence
results in each geographic location before incorporating the test into
routine diagnostic work.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.10.3736-3739.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Molecular Characterization of Rifampin-Resistant
Isolates of Mycobacterium tuberculosis from Hungary by
DNA Sequencing and the Line Probe Assay
*
Corresponding author. Mailing address: Wadsworth
Center, New York State Department of Health, P.O. Box 509, Albany, NY
12201-0509. Phone: (518) 474-2196. Fax: (518) 474-6964. E-mail:
somoskov{at}wadsworth.org or medve{at}pulm.sote.hu.
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