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Journal of Clinical Microbiology, October 2001, p. 3750-3752, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3750-3752.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Removal of PCR Inhibitors by Silica Membranes: Evaluating the Amplicor Mycobacterium tuberculosis Kit

Boris Böddinghaus,* Thomas A. Wichelhaus, Volker Brade, and Thomas Bittner

Institute of Medical Microbiology, University Hospital, Frankfurt am Main, Germany

Received 2 April 2001/Returned for modification 2 May 2001/Accepted 15 July 2001

The effectiveness of PCR inhibitor removal by silica membranes in combination with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 respiratory and nonrespiratory specimens. The overall inhibition rate was reduced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the addition of silica membrane DNA purification.

* Corresponding author. Mailing address: Institute of Medical Microbiology, University Hospital, Paul-Ehrlich-Str. 40, 60596 Frankfurt am Main, Germany. Phone: 49/69/6301-5019. Fax: 49/69/6301-5767. E-mail: Boeddinghaus{at}em.uni-frankfurt.de.

Journal of Clinical Microbiology, October 2001, p. 3750-3752, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3750-3752.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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