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Journal of Clinical Microbiology, November 2001, p. 3858-3864, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.3858-3864.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Correlation between Mutations in the Interferon Sensitivity-Determining Region of NS5A Protein and Viral Load of Hepatitis C Virus Subtypes 1b, 1c, and 2a

Maria I. Lusida,1,2 Motoko Nagano-Fujii,3 Chairul A. Nidom,2,4 Soetjipto,2,4 Retno Handajani,2,4 Tsunenori Fujita,3 Kiyomasa Oka,3 and Hak Hotta3,5,*

Departments of Microbiology1 and Biochemistry,4 Faculty of Medicine, and Tropical Disease Center,2 Airlangga University, Surabaya, Indonesia, and Department of Microbiology3 and International Center for Medical Research,5 Kobe University Graduate School of Medicine, Kobe, Japan

Received 9 March 2001/Returned for modification 11 June 2001/Accepted 1 August 2001

In the present study, we analyzed the possible relationship between interferon (IFN) sensitivity-determining region (ISDR) sequence variation of various hepatitis C virus (HCV) subtypes and serum HCV titers in Indonesian patients without IFN treatment. The viremia titers (mean ± standard deviation) of HCV subtype 1b (HCV-1b) isolates with low (three or fewer) and high (four or more) numbers of ISDR mutations were 5.4 ± 0.6 and 4.2 ± 0.9 log10 RNA copies/ml, respectively, with the difference between the two groups being statistically significant (P < 0.01). Similarly, the viremia titers of HCV-1c isolates with low and high numbers of ISDR mutations were 5.3 ± 0.6 and <3.0 ± 0.0 log10 RNA copies/ml, respectively, with the difference between the two groups being statistically significant (P < 0.01). Also, the virus titers of HCV-2a isolates with low and high numbers of ISDR mutations were 4.3 ± 0.7 and 3.5 ± 0.4 log10 RNA copies/ml, respectively, with the difference between the two groups being statistically significant (P < 0.01). Thus, our results demonstrated that virus load in Indonesian patients infected with HCV-1b, HCV-1c, or HCV-2a correlated inversely with the number of mutations in the ISDR sequence, implying the possibility that the ISDR sequence plays an important role in determining the levels of HCV viremia.

* Corresponding author. Mailing address: Department of Microbiology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. Phone: 81-78-382-5500. Fax: 81-78-382-5519. E-mail: hotta{at}kobe-u.ac.jp.

Journal of Clinical Microbiology, November 2001, p. 3858-3864, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.3858-3864.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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