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Journal of Clinical Microbiology, November 2001, p. 3915-3919, Vol. 39, No. 11
Department of Clinical Microbiology and
Immunology1 and Outpatient Sexually
Transmitted Disease Clinic, Department of
Dermatovenereology,2 Örebro Medical Centre
Hospital, SE-70185 Örebro, Department of Clinical
Microbiology, Malmö University Hospital, SE-20502
Malmö,3 and Division of Virology,
Department of Microbiology, Pathology and Immunology, Karolinska
Institutet, Huddinge University Hospital, SE-14186
Huddinge/Stockholm,4 Sweden
Received 16 April 2001/Returned for modification 15 June
2001/Accepted 12 August 2001
A method for detection and genotyping of genital Chlamydia
trachomatis infections based on omp1 gene
amplification and sequencing was developed. DNA was extracted from
urogenital or urine samples using a Chelex-based method, and an
approximately 1,100-bp-long fragment from the omp1 gene
was directly amplified and sequenced. Genotyping was performed by BLAST
similarity search, and phylogenetic tree analysis was used to
illustrate the evolutionary relationships between clinical isolates and
reference strains. The method was used to determine the genotypes of
C. trachomatis in 237 positive urogenital and/or urine
specimens collected at a Swedish sexually transmitted disease clinic
during 1 year. The most common genotypes corresponded to serotypes E
(47%) and F (17%). The omp1 gene was highly conserved
for genotype E (106 of 112 samples without any mutation) and F (41 of
42 samples without any mutation) strains but appear slightly less
conserved for genotypes G (n = 6) and H
(n = 6), where the sequences displayed one to four
nucleotide substitutions relative to the reference sequence. Genotyping
of samples collected at the follow-up visit indicated that two patients had become reinfected, while three other patients suffered treatment failure or reinfection. One woman appeared to have a mixed infection with two different C. trachomatis strains. This
omp1 genotyping method had a high reproducibility and
could be used for epidemiological characterization of sexually
transmitted Chlamydia infections.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.11.3915-3919.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Characterization of Chlamydia trachomatis
omp1 Genotypes among Sexually Transmitted Disease Patients
in Sweden
*
Corresponding author. Mailing address: Department of
Clinical Microbiology and Immunology, Örebro Medical Centre
Hospital, SE-70185 Örebro, Sweden. Phone: 46-19-6021520. Fax:
46-19-127416. E-mail: margaretha.jurstrand{at}orebroll.se.
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