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Journal of Clinical Microbiology, November 2001, p. 3955-3961, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.3955-3961.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Phenotypic Identification of Actinomyces and Related Species Isolated from Human Sources

Nanna Sarkonen,1,* Eija Könönen,1 Paula Summanen,2 Mauno Könönen,3,4,5 and Hannele Jousimies-Somer1

Anaerobe Reference Laboratory, National Public Health Institute,1 University of Helsinki,4 and Helsinki University Central Hospital,5 Helsinki, Finland; Veterans Affairs Wadsworth Medical Center, Los Angeles, California2; and University of Aarhus, Aarhus, Denmark3

Received 8 May 2001/Returned for modification 23 July 2001/Accepted 21 August 2001

Recent advancements in chemotaxonomic and molecular biology-based identification methods have clarified the taxonomy of the genus Actinomyces and have led to the recognition of several new Actinomyces and related species. Actinomyces-like gram-positive rods have increasingly been isolated from various clinical specimens. Thus, an easily accessible scheme for reliable differentiation at the species level is needed in clinical and oral microbiology laboratories, where bacterial identification is mainly based on conventional biochemical methods. In the present study we designed a two-step protocol that consists of a flowchart that describes rapid, cost-efficient tests for preliminary identification of Actinomyces and closely related species and an updated more comprehensive scheme that also uses fermentation reactions for accurate differentiation of Actinomyces and closely related species.

* Corresponding author. Mailing address: Anaerobe Reference Laboratory, Department of Microbiology, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland. Phone: 358-9-47448254. Fax: 358-9-47448238. E-mail: nanna.sarkonen{at}ktl.fi.

Journal of Clinical Microbiology, November 2001, p. 3955-3961, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.3955-3961.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.