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Journal of Clinical Microbiology, November 2001, p. 4032-4036, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4032-4036.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Pulsed-Field Gel Electrophoresis in Differentiation of Erysipelothrix Species Strains

Alexandre Tomomitsu Okatani, Takehiko Uto, Takahide Taniguchi, Tomoko Horisaka, Tetsuya Horikita, Ken-Ichi Kaneko, and Hideki Hayashidani*

Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan

Received 29 May 2001/Returned for modification 16 July 2001/Accepted 3 September 2001

We report here the first analysis of Erysipelothrix spp. using pulsed-field gel electrophoresis (PFGE). Seventy strains of Erysipelothrix spp. were analyzed. SmaI, AscI, and NotI were tested for the ability to cleave the DNA extracted from those strains, and among them, SmaI was the most reliable enzyme. Sixty-three distinct PFGE patterns were produced, and no DNA degradation was observed, allowing the identification of all of the strains. Based on these results and on those of a previous analysis using randomly amplified polymorphic DNA and ribotyping, PFGE with SmaI might be considered to be more sensitive than those methods and to be the best method for epidemiological studies of strains of this genus.

* Corresponding author. Mailing address: Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan. Phone: 81-42-367-5775. Fax: 81-42-360-8830. E-mail: eisei{at}cc.tuat.ac.jp.

Journal of Clinical Microbiology, November 2001, p. 4032-4036, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4032-4036.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nagai, S., To, H., Kanda, A. (2008). Differentiation of Erysipelothrix rhusiopathiae strains by nucleotide sequence analysis of a hypervariable region in the spaA gene: discrimination of a live vaccine strain from field isolates. jvdi 20: 336-342 [Abstract] [Full Text]  


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