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Journal of Clinical Microbiology, November 2001, p. 4082-4085, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4082-4085.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Chlamydia trachomatis Serology: Diagnostic Value of Outer Membrane Protein 2 Compared with That of Other Antigens

S. Bas,1,* P. Muzzin,2 and T. L. Vischer1

Division of Rheumatology, Department of Internal Medicine, University Hospital,1 and Medical Biochemistry Department, Geneva Medical School,2 1211 Geneva 14, Switzerland

Received 21 February 2001/Returned for modification 23 July 2001/Accepted 31 August 2001

Different immunoassays using recombinant antigens or synthetic peptides were evaluated for the serodiagnosis of Chlamydia trachomatis infections. Antigens used included cysteine-rich outer membrane protein 2 (OMP2), heat shock protein 60, the polypeptide encoded by open reading frame 3 of the plasmid (pgp3), synthetic peptides derived from species-specific epitopes in variable domain IV of the major OMP (MOMP) (Labsystems, Helsinki, Finland), and a fragment of the total lipopolysaccharide (Medac, Hamburg, Germany). Because cross-reactions between chlamydial species have been reported, Chlamydia pneumoniae-specific antibodies were also determined by immunoassays (Labsystems). Responses obtained with serum samples from patients with well-defined diseases (i.e., urethral or endocervical samples from which C. trachomatis DNA was amplified) were compared to those obtained with samples from healthy blood donors. The best sensitivity (79%) associated with the best specificity (82%) was obtained when immunoglobulin G (IgG) responses to both MOMP and pgp3 were considered. The highest sensitivity (89%) was obtained with anti-OMP2 IgG, but the lowest specificity (57%) was obtained with this antibody, due to probable cross-reactivity with C. pneumoniae OMP2.

* Corresponding author. Mailing address: Division of Rheumatology, Department of Internal Medicine, University Hospital, 1211 Geneva 14, Switzerland. Phone: (41 22) 382 36 80. Fax: (41 22) 382 35 30. E-mail: bas-sylvette{at}diogenes.hcuge.ch.

Journal of Clinical Microbiology, November 2001, p. 4082-4085, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4082-4085.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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