Use of Quantitative PCR To Measure Density of Borrelia burgdorferi in the Midgut and Salivary Glands of Feeding Tick Vectors

doi:10.1128/JCM.39.11.4145-4148.2001

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Journal of Clinical Microbiology, November 2001, p. 4145-4148, Vol. 39, No. 11
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.11.4145-4148.2001

Use of Quantitative PCR To Measure Density of Borrelia burgdorferi in the Midgut and Salivary Glands of Feeding Tick Vectors

Joseph Piesman,^* Bradley S. Schneider, and Nordin S. Zeidner

Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Public Health Service, Fort Collins, Colorado 80522

Received 2 April 2001/Returned for modification 29 July 2001/Accepted 20 August 2001

Quantitative real-time PCR was used to assay spirochetes in feeding ticks. Spirochetes in tick midguts increased sixfold,from 998 per tick before attachment to 5,884 at 48 h of attachment.Spirochetes in tick salivary glands increased >17-fold, from 1.2per salivary gland pair before feeding to 20.8 at 72 h postattachment.The period of the most rapid increase in the number of spirochetesin the salivary glands occurred from 48 to 60 h postattachment;this time period coincides with the maximal increase in transmissionrisk during nymphal tickfeeding.

^* Corresponding author. Mailing address: CDC/DVBID, P.O. Box 2087, Fort Collins, CO 80522. Phone: (970) 221-6408. Fax: (970)221-6476. E-mail: JPiesman{at}cdc.gov.

Journal of Clinical Microbiology, November 2001, p. 4145-4148, Vol. 39, No. 11
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.11.4145-4148.2001

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